3cqa: Difference between revisions

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-[[Image:3cqa.png|left|200px]]
-{{STRUCTURE_3cqa|  PDB=3cqa  |  SCENE=  }}
+==Crystal structure of human fibroblast growth factor-1 with mutations Glu81Ala and Lys101Ala==
+<StructureSection load='3cqa' size='340' side='right'caption='[[3cqa]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3cqa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CQA FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cqa OCA], [https://pdbe.org/3cqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cqa RCSB], [https://www.ebi.ac.uk/pdbsum/3cqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cqa ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FGF1_HUMAN FGF1_HUMAN] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:8663044</ref> <ref>PMID:16597617</ref> <ref>PMID:20145243</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/3cqa_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cqa ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Large-volume protein crystals are a prerequisite for neutron diffraction studies and their production represents a bottleneck in obtaining neutron structures. Many protein crystals that permit the collection of high-resolution X-ray diffraction data are inappropriate for neutron diffraction owing to a plate-type morphology that limits the crystal volume. Human fibroblast growth factor 1 crystallizes in a plate morphology that yields atomic resolution X-ray diffraction data but has insufficient volume for neutron diffraction. The thin physical dimension has been identified as corresponding to the b cell edge and the X-ray structure identified a solvent-mediated crystal contact adjacent to position Glu81 that was hypothesized to limit efficient crystal growth in this dimension. In this report, a series of mutations at this crystal contact designed to both reduce side-chain entropy and replace the solvent-mediated interface with direct side-chain contacts are reported. The results suggest that improved crystal growth is achieved upon the introduction of direct crystal contacts, while little improvement is observed with side-chain entropy-reducing mutations alone.
-===Crystal structure of human fibroblast growth factor-1 with mutations Glu81Ala and Lys101Ala===
+Engineering an improved crystal contact across a solvent-mediated interface of human fibroblast growth factor 1.,Meher AK, Blaber SI, Lee J, Honjo E, Kuroki R, Blaber M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt, 11):1136-40. Epub 2009 Oct 30. PMID:19923735<ref>PMID:19923735</ref>
-{{ABSTRACT_PUBMED_19923735}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3cqa" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[3cqa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CQA OCA].
+*[[Fibroblast growth factor 3D structures|Fibroblast growth factor 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019923735</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Blaber, M.]]
+[[Category: Large Structures]]
-[[Category: Honjo, E.]]
+[[Category: Blaber M]]
-[[Category: Kuroki, R.]]
+[[Category: Honjo E]]
-[[Category: Lee, J.]]
+[[Category: Kuroki R]]
-[[Category: Meher, A K.]]
+[[Category: Lee J]]
-[[Category: Somasundaram, T.]]
+[[Category: Meher AK]]
-[[Category: Angiogenesis]]
+[[Category: Somasundaram T]]
-[[Category: Crystal packing]]
-[[Category: Developmental protein]]
-[[Category: Differentiation]]
-[[Category: Growth factor]]
-[[Category: Heparin-binding]]
-[[Category: Hormone]]
-[[Category: Mitogen]]