3ct5: Difference between revisions

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[[Image:3ct5.png|left|200px]]


{{STRUCTURE_3ct5|  PDB=3ct5  |  SCENE=  }}
==Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail==
 
<StructureSection load='3ct5' size='340' side='right'caption='[[3ct5]], [[Resolution|resolution]] 1.37&Aring;' scene=''>
===Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3ct5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_phi29 Bacillus phage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CT5 FirstGlance]. <br>
{{ABSTRACT_PUBMED_18606992}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.37&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900017:triacetyl-beta-chitotriose'>PRD_900017</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ct5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ct5 OCA], [https://pdbe.org/3ct5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ct5 RCSB], [https://www.ebi.ac.uk/pdbsum/3ct5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ct5 ProSAT]</span></td></tr>
[[3ct5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_phi29 Bacillus phage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CT5 OCA].  
</table>
 
== Function ==
==Reference==
[https://www.uniprot.org/uniprot/GP13_BPPH2 GP13_BPPH2] May serve as a plug to restrain the highly pressurized packaged genome and thus would be the first virion protein to contact the host cell wall, degrading the peptidoglycan layer and thereby facilitating viral genome entry into the host bacteria. Acts probably as a multifunctional enzyme that degrades N-acetylglucosamine polymers (in vitro) and cleaves the peptide cross-links of the host cell wall. Essential for the tail assembly.<ref>PMID:18394643</ref> <ref>PMID:18606992</ref>
<ref group="xtra">PMID:018606992</ref><references group="xtra"/>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/3ct5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ct5 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus phage phi29]]
[[Category: Bacillus phage phi29]]
[[Category: Rossmann, M G.]]
[[Category: Large Structures]]
[[Category: Xiang, Y.]]
[[Category: Rossmann MG]]
[[Category: Cell wall]]
[[Category: Xiang Y]]
[[Category: Hydrolase]]
[[Category: Infection]]
[[Category: Late protein]]
[[Category: Phi29]]

Latest revision as of 12:38, 21 February 2024

Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tailCrystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail

Structural highlights

3ct5 is a 1 chain structure with sequence from Bacillus phage phi29. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.37Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GP13_BPPH2 May serve as a plug to restrain the highly pressurized packaged genome and thus would be the first virion protein to contact the host cell wall, degrading the peptidoglycan layer and thereby facilitating viral genome entry into the host bacteria. Acts probably as a multifunctional enzyme that degrades N-acetylglucosamine polymers (in vitro) and cleaves the peptide cross-links of the host cell wall. Essential for the tail assembly.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Cohen DN, Erickson SE, Xiang Y, Rossmann MG, Anderson DL. Multifunctional roles of a bacteriophage phi 29 morphogenetic factor in assembly and infection. J Mol Biol. 2008 May 9;378(4):804-17. doi: 10.1016/j.jmb.2008.02.068. Epub 2008, Mar 7. PMID:18394643 doi:http://dx.doi.org/10.1016/j.jmb.2008.02.068
  2. ↑ Xiang Y, Morais MC, Cohen DN, Bowman VD, Anderson DL, Rossmann MG. Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail. Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9552-7. Epub 2008 Jul 7. PMID:18606992

3ct5, resolution 1.37Å

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