1wl0: Difference between revisions

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-[[Image:1wl0.gif|left|200px]]<br /><applet load="1wl0" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1wl0, resolution 3.2&Aring;" />
-'''Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima R44A mutant'''<br />
-==Overview==
+==Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima R44A mutant==
-Octaprenyl pyrophosphate synthase (OPPs) catalyzes the condensation of five isopentenyl pyrophosphates with farnesyl pyrophosphate to generate C(40) octaprenyl pyrophosphate. The enzymes from the hyperthermophilic bacterium Thermotoga maritima and from the mesophilic Escherichia coli were expressed in E. coli and the recombinant proteins were purified and crystallized. The T. maritima OPPs crystals belong to space group P42(1)2, with unit-cell parameters a = b = 151.53, c = 69.72 A. The E. coli OPPs crystals belong to space group C222(1), with unit-cell parameters a = 247.66, b = 266.10, c = 157.93 A. Diffraction data were collected at 100 K using synchrotron radiation and an in-house X-ray source. Structure determination of T. maritima OPPs has been carried out using MIR data sets at 2.8 A resolution. The asymmetric unit contains one dimer. An initial model with 280 residues per subunit has been built and refined to 2.28 A resolution. It shows mostly helical structure and resembles that of avian farnesyl pyrophosphate synthase.
+<StructureSection load='1wl0' size='340' side='right'caption='[[1wl0]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1wl0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WL0 FirstGlance]. <br>
-WL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima_msb8 Thermotoga maritima msb8] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Trans-octaprenyltranstransferase Trans-octaprenyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.11 2.5.1.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WL0 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wl0 OCA], [https://pdbe.org/1wl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wl0 RCSB], [https://www.ebi.ac.uk/pdbsum/1wl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wl0 ProSAT]</span></td></tr>
-Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli., Guo RT, Ko TP, Chou CC, Shr HL, Chu HM, Tsai YH, Liang PH, Wang AH, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2265-8. Epub 2003, Nov 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14646090 14646090]
+</table>
-[[Category: Single protein]]
+== Function ==
-[[Category: Thermotoga maritima msb8]]
+[https://www.uniprot.org/uniprot/Q9X1M1_THEMA Q9X1M1_THEMA]
-[[Category: Trans-octaprenyltranstransferase]]
+== Evolutionary Conservation ==
-[[Category: Cheng, Y L.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Cheng, Y S.]]
+Check<jmol>
-[[Category: Guo, R T.]]
+  <jmolCheckbox>
-[[Category: Kuo, C J.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/1wl0_consurf.spt"</scriptWhenChecked>
-[[Category: Liang, P H.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Wang, A H.J.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: SO4]]
+  </jmolCheckbox>
-[[Category: thermophilic]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wl0 ConSurf].
-[[Category: trans-type prenyltransferase]]
+<div style="clear:both"></div>
+__TOC__
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:45:28 2008''
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Thermotoga maritima MSB8]]
+[[Category: Cheng YL]]
+[[Category: Cheng YS]]
+[[Category: Guo RT]]
+[[Category: Kuo CJ]]
+[[Category: Liang PH]]
+[[Category: Wang AH-J]]