2cut: Difference between revisions

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-[[Image:2cut.png|left|200px]]
-{{STRUCTURE_2cut|  PDB=2cut  |  SCENE=  }}
+==CUTINASE, A LIPOLYTIC ENZYME WITH A PREFORMED OXYANION HOLE==
+<StructureSection load='2cut' size='340' side='right'caption='[[2cut]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2cut]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_vanettenii Fusarium vanettenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CUT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CUT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cut OCA], [https://pdbe.org/2cut PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cut RCSB], [https://www.ebi.ac.uk/pdbsum/2cut PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cut ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/2cut_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cut ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cutinases, a group of cutin degrading enzymes with molecular masses of around 22-25 kDa (Kolattukudy, 1984), are also able to efficiently hydrolyse triglycerides (De Geus et al., 1989; Lauwereys et al., 1991), but without exhibiting the interfacial activation phenomenom (Sarda et al., 1958). They belong to a class of proteins with a common structural framework, called the alpha/beta hydrolase fold (Martinez et al., 1992; Ollis et al., 1992). We describe herein the structure of cutinase covalently inhibited by diethyl-p-nitrophenyl phosphate (E600) and refined at 1.9-A resolution. Contrary to what has previously been reported with lipases (Brzozowski et al., 1991; Derewenda et al., 1992; Van Tilbeurgh et al., 1993), no significant structural rearrangement was observed here in cutinase upon the inhibitor binding. Moreover, the structure of the active site machinery, consisting of a catalytic triad (S120, H188, D175) and an oxyanion hole (Q121 and S42), was found to be identical to that of the native enzyme, whereas the oxyanion hole of Rhizomucor lipase (Brzozowski et al., 1991; Derewenda et al., 1992), like that of pancreatic lipase (van Tilbeurgh et al., 1993), is formed only upon enzyme-ligand complex formation. The fact that cutinase does not display interfacial activation cannot therefore only be due to the absence of a lid but might also be attributable to the presence of a preformed oxyanion hole.
-===CUTINASE, A LIPOLYTIC ENZYME WITH A PREFORMED OXYANION HOLE===
+Cutinase, a lipolytic enzyme with a preformed oxyanion hole.,Martinez C, Nicolas A, van Tilbeurgh H, Egloff MP, Cudrey C, Verger R, Cambillau C Biochemistry. 1994 Jan 11;33(1):83-9. PMID:8286366<ref>PMID:8286366</ref>
-{{ABSTRACT_PUBMED_8286366}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2cut" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[2cut]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani Fusarium solani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CUT OCA].
+*[[Cutinase 3D structures|Cutinase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:008286366</ref><references group="xtra"/>
+__TOC__
-[[Category: Fusarium solani]]
+</StructureSection>
-[[Category: Cambillau, C.]]
+[[Category: Fusarium vanettenii]]
-[[Category: Martinez, C.]]
+[[Category: Large Structures]]
+[[Category: Cambillau C]]
+[[Category: Martinez C]]