1ul9: Difference between revisions

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-[[Image:1ul9.gif|left|200px]]<br /><applet load="1ul9" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1ul9, resolution 2.22&Aring;" />
-'''CGL2 ligandfree'''<br />
-==Overview==
+==CGL2 ligandfree==
-Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted oligosaccharides of the mammalian blood group A/B type in an extended binding cleft. Kinetic studies on wild-type and mutant CGL2 proteins demonstrate that the tetrameric organization is essential for functionality. The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity.
+<StructureSection load='1ul9' size='340' side='right'caption='[[1ul9]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ul9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UL9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ul9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ul9 OCA], [https://pdbe.org/1ul9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ul9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ul9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ul9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CGL2_COPCI CGL2_COPCI] Binds lactose. May play a role in fruiting body formation.<ref>PMID:8999822</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ul/1ul9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ul9 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-UL9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UL9 OCA].
+*[[Galectin 3D structures|Galectin 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structure and functional analysis of the fungal galectin CGL2., Walser PJ, Haebel PW, Kunzler M, Sargent D, Kues U, Aebi M, Ban N, Structure. 2004 Apr;12(4):689-702. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15062091 15062091]
+__TOC__
+</StructureSection>
 [[Category: Coprinopsis cinerea]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Aebi, M.]]
+[[Category: Aebi M]]
-[[Category: Ban, N.]]
+[[Category: Ban N]]
-[[Category: Haebel, P W.]]
+[[Category: Haebel PW]]
-[[Category: Kuenzler, M.]]
+[[Category: Kuenzler M]]
-[[Category: Kues, U.]]
+[[Category: Kues U]]
-[[Category: Walser, P J.]]
+[[Category: Walser PJ]]
-[[Category: beta-galactoside binding lectin]]
-[[Category: galectin]]
-[[Category: lectin]]
-[[Category: sugar binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:49 2008''