1tv3: Difference between revisions

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-[[Image:1tv3.png|left|200px]]
-{{STRUCTURE_1tv3|  PDB=1tv3  |  SCENE=  }}
+==Crystal structure of the N-methyl-hydroxylamine MtmB complex==
+<StructureSection load='1tv3' size='340' side='right'caption='[[1tv3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1tv3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TV3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BG4:5-(HYDROXY-METHYL-AMINO)-3-METHYL-PYRROLIDINE-2-CARBOXYLIC+ACID'>BG4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tv3 OCA], [https://pdbe.org/1tv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tv3 RCSB], [https://www.ebi.ac.uk/pdbsum/1tv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tv3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MTMB1_METBA MTMB1_METBA] Catalyzes the transfer of the methyl group from monomethylamine to the corrinoid cofactor of MtmC (MtmC1 or MtmC2).<ref>PMID:9642198</ref> <ref>PMID:9195968</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.
-===Crystal structure of the N-methyl-hydroxylamine MtmB complex===
+Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid.,Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK Chem Biol. 2004 Sep;11(9):1317-24. PMID:15380192<ref>PMID:15380192</ref>
-{{ABSTRACT_PUBMED_15380192}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1tv3" style="background-color:#fffaf0;"></div>
-[[1tv3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TV3 OCA].
+== References ==
+<references/>
-==See Also==
+__TOC__
-*[[Amino Acids|Amino Acids]]
+</StructureSection>
+[[Category: Large Structures]]
-==Reference==
-<ref group="xtra">PMID:015380192</ref><references group="xtra"/>
 [[Category: Methanosarcina barkeri]]
-[[Category: Chan, M K.]]
+[[Category: Chan MK]]
-[[Category: Ferguson, T K.]]
+[[Category: Ferguson TK]]
-[[Category: Gallucci, J.]]
+[[Category: Gallucci J]]
-[[Category: Hao, B.]]
+[[Category: Hao B]]
-[[Category: Kang, P T.]]
+[[Category: Kang PT]]
-[[Category: Krzycki, J A.]]
+[[Category: Krzycki JA]]
-[[Category: Soares, J A.]]
+[[Category: Soares JA]]
-[[Category: Zhao, G.]]
+[[Category: Zhao G]]
-[[Category: Tim barrel]]
-[[Category: Transferase]]