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[[Image:1tzc.jpg|left|200px]]<br /><applet load="1tzc" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1tzc, resolution 1.45&Aring;" />
'''Crystal structure of phosphoglucose/phosphomannose isomerase from Pyrobaculum aerophilum in complex with 5-phosphoarabinonate'''<br />


==Overview==
==Crystal structure of phosphoglucose/phosphomannose isomerase from Pyrobaculum aerophilum in complex with 5-phosphoarabinonate==
The crystal structure of a dual specificity phosphoglucose isomerase (PGI)/phosphomannose isomerase from Pyrobaculum aerophilum (PaPGI/PMI) has been determined in native form at 1.16-A resolution and in complex with the enzyme inhibitor 5-phosphoarabinonate at 1.45-A resolution. The similarity of its fold, with the inner core structure of PGIs from eubacterial and eukaryotic sources, confirms this enzyme as a member of the PGI superfamily. The almost total conservation of amino acids in the active site, including the glutamate base catalyst, shows that PaPGI/PMI uses the same catalytic mechanisms for both ring opening and isomerization for the interconversion of glucose 6-phosphate (Glc-6-P) to fructose 6-phosphate (Fru-6-P). The lack of structural differences between native and inhibitor-bound enzymes suggests this activity occurs without any of the conformational changes that are the hallmark of the well characterized PGI family. The lack of a suitable second base in the active site of PaPGI/PMI argues against a PMI mechanism involving a trans-enediol intermediate. Instead, PMI activity may be the result of additional space in the active site imparted by a threonine, in place of a glutamine in other PGI enzymes, which could permit rotation of the C-2-C-3 bond of mannose 6-phosphate.
<StructureSection load='1tzc' size='340' side='right'caption='[[1tzc]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tzc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrobaculum_aerophilum_str._IM2 Pyrobaculum aerophilum str. IM2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TZC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PA5:5-PHOSPHOARABINONIC+ACID'>PA5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzc OCA], [https://pdbe.org/1tzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tzc RCSB], [https://www.ebi.ac.uk/pdbsum/1tzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tzc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PGMI_PYRAE PGMI_PYRAE] Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tz/1tzc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tzc ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1TZC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum_str._im2 Pyrobaculum aerophilum str. im2] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=PA5:'>PA5</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZC OCA].
*[[Phosphoglucose isomerase 3D structures|Phosphoglucose isomerase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
A novel phosphoglucose isomerase (PGI)/phosphomannose isomerase from the crenarchaeon Pyrobaculum aerophilum is a member of the PGI superfamily: structural evidence at 1.16-A resolution., Swan MK, Hansen T, Schonheit P, Davies C, J Biol Chem. 2004 Sep 17;279(38):39838-45. Epub 2004 Jul 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15252053 15252053]
[[Category: Large Structures]]
[[Category: Pyrobaculum aerophilum str. im2]]
[[Category: Pyrobaculum aerophilum str. IM2]]
[[Category: Single protein]]
[[Category: Davies C]]
[[Category: Davies, C.]]
[[Category: Hansen T]]
[[Category: Hansen, T.]]
[[Category: Schoenheit P]]
[[Category: Schoenheit, P.]]
[[Category: Swan MK]]
[[Category: Swan, M K.]]
[[Category: GOL]]
[[Category: PA5]]
[[Category: SO4]]
[[Category: crenarchaeon]]
[[Category: enzyme]]
[[Category: hyperthermophile]]
[[Category: pgi family]]
 
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