2m22: Difference between revisions
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==Solution structure of the helix II template boundary element from Tetrahymena telomerase RNA== | |||
<StructureSection load='2m22' size='340' side='right'caption='[[2m22]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2m22]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2frl 2frl]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M22 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m22 OCA], [https://pdbe.org/2m22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m22 RCSB], [https://www.ebi.ac.uk/pdbsum/2m22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m22 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Telomere addition by telomerase requires an internal templating sequence located in the RNA subunit of telomerase. The correct boundary definition of this template sequence is essential for the proper addition of the nucleotide repeats. Incorporation of incorrect telomeric repeats onto the ends of chromosomes has been shown to induce chromosomal instability in ciliate, yeast and human cells. A 5' template boundary defining element (TBE) has been identified in human, yeast and ciliate telomerase RNAs. Here, we report the solution structure of the TBE element (helix II) from Tetrahymena thermophila telomerase RNA. Our results indicate that helix II and its capping pentaloop form a well-defined structure including unpaired, stacked adenine nucleotides in the stem and an unusual syn adenine nucleotide in the loop. A comparison of the T.thermophila helix II pentaloop with a pentaloop of the same sequence found in the 23S rRNA of the Haloarcula marismortui ribosome suggests possible RNA and/or protein interactions for the helix II loop within the Tetrahymena telomerase holoenzyme. | |||
Structure of the Tetrahymena thermophila telomerase RNA helix II template boundary element.,Richards RJ, Theimer CA, Finger LD, Feigon J Nucleic Acids Res. 2006 Feb 1;34(3):816-25. Print 2006. PMID:16452301<ref>PMID:16452301</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2m22" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Telomerase 3D structures|Telomerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Cash DD]] | |||
[[Category: Feigon J]] | |||
[[Category: Finger DL]] | |||
[[Category: Richards RJ]] | |||
[[Category: Theimer CA]] | |||
Latest revision as of 08:57, 15 May 2024
Solution structure of the helix II template boundary element from Tetrahymena telomerase RNASolution structure of the helix II template boundary element from Tetrahymena telomerase RNA
Structural highlights
Publication Abstract from PubMedTelomere addition by telomerase requires an internal templating sequence located in the RNA subunit of telomerase. The correct boundary definition of this template sequence is essential for the proper addition of the nucleotide repeats. Incorporation of incorrect telomeric repeats onto the ends of chromosomes has been shown to induce chromosomal instability in ciliate, yeast and human cells. A 5' template boundary defining element (TBE) has been identified in human, yeast and ciliate telomerase RNAs. Here, we report the solution structure of the TBE element (helix II) from Tetrahymena thermophila telomerase RNA. Our results indicate that helix II and its capping pentaloop form a well-defined structure including unpaired, stacked adenine nucleotides in the stem and an unusual syn adenine nucleotide in the loop. A comparison of the T.thermophila helix II pentaloop with a pentaloop of the same sequence found in the 23S rRNA of the Haloarcula marismortui ribosome suggests possible RNA and/or protein interactions for the helix II loop within the Tetrahymena telomerase holoenzyme. Structure of the Tetrahymena thermophila telomerase RNA helix II template boundary element.,Richards RJ, Theimer CA, Finger LD, Feigon J Nucleic Acids Res. 2006 Feb 1;34(3):816-25. Print 2006. PMID:16452301[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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