1oeb: Difference between revisions

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-[[Image:1oeb.png|left|200px]]
-{{STRUCTURE_1oeb|  PDB=1oeb  |  SCENE=  }}
+==Mona/Gads SH3C domain==
+<StructureSection load='1oeb' size='340' side='right'caption='[[1oeb]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1oeb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OEB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OEB FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oeb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oeb OCA], [https://pdbe.org/1oeb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oeb RCSB], [https://www.ebi.ac.uk/pdbsum/1oeb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oeb ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GRAP2_MOUSE GRAP2_MOUSE] Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oe/1oeb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oeb ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+SH3 domains are protein recognition modules within many adaptors and enzymes. With more than 500 SH3 domains in the human genome, binding selectivity is a key issue in understanding the molecular basis of SH3 domain interactions. The Grb2-like adaptor protein Mona/Gads associates stably with the T-cell receptor signal transducer SLP-76. The crystal structure of a complex between the C-terminal SH3 domain (SH3C) of Mona/Gads and a SLP-76 peptide has now been solved to 1.7 A. The peptide lacks the canonical SH3 domain binding motif P-x-x-P and does not form a frequently observed poly-proline type II helix. Instead, it adopts a clamp-like shape around the circumfence of the SH3C beta-barrel. The central R-x-x-K motif of the peptide forms a 3(10) helix and inserts into a negatively charged double pocket on the SH3C while several other residues complement binding through hydrophobic interactions, creating a short linear SH3C binding epitope of uniquely high affinity. Interestingly, the SH3C displays ion-dependent dimerization in the crystal and in solution, suggesting a novel mechanism for the regulation of SH3 domain functions.
-===MONA/GADS SH3C DOMAIN===
+Structural basis for SH3 domain-mediated high-affinity binding between Mona/Gads and SLP-76.,Harkiolaki M, Lewitzky M, Gilbert RJ, Jones EY, Bourette RP, Mouchiroud G, Sondermann H, Moarefi I, Feller SM EMBO J. 2003 Jun 2;22(11):2571-82. PMID:12773374<ref>PMID:12773374</ref>
-{{ABSTRACT_PUBMED_12773374}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1oeb" style="background-color:#fffaf0;"></div>
-[[1oeb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OEB OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:012773374</ref><references group="xtra"/>
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Bourette, R P.]]
+[[Category: Bourette RP]]
-[[Category: Feller, S M.]]
+[[Category: Feller SM]]
-[[Category: Gilbert, R J.C.]]
+[[Category: Gilbert RJC]]
-[[Category: Harkiolaki, M.]]
+[[Category: Harkiolaki M]]
-[[Category: Jones, E Y.]]
+[[Category: Jones EY]]
-[[Category: Lewitzky, M.]]
+[[Category: Lewitzky M]]
-[[Category: Moarefi, I.]]
+[[Category: Moarefi I]]
-[[Category: Mouchiroud, G.]]
+[[Category: Mouchiroud G]]
-[[Category: Sondermann, H.]]
+[[Category: Sondermann H]]
-[[Category: Dimer]]
-[[Category: Gad]]
-[[Category: Mona]]
-[[Category: Protein binding]]
-[[Category: Sh3]]
-[[Category: Sh3 domain-complex]]
-[[Category: Signal tranduction]]
-[[Category: Slp-76]]