1mj5: Difference between revisions

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[[Image:1mj5.png|left|200px]]


{{STRUCTURE_1mj5| PDB=1mj5 | SCENE= }}
==LINB (haloalkane dehalogenase) from sphingomonas paucimobilis UT26 at atomic resolution==
<StructureSection load='1mj5' size='340' side='right'caption='[[1mj5]], [[Resolution|resolution]] 0.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mj5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJ5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mj5 OCA], [https://pdbe.org/1mj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mj5 RCSB], [https://www.ebi.ac.uk/pdbsum/1mj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mj5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LINB_SPHJU LINB_SPHJU] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity since not only monochloroalkanes (C3 to C10) but also dichloroalkanes (> C3), bromoalkanes, and chlorinated aliphatic alcohols are good substrates (PubMed:9293022, PubMed:10100638). Shows almost no activity with 1,2-dichloroethane, but very high activity with the brominated analog (PubMed:9293022). Is involved in the degradation of the important environmental pollutant gamma-hexachlorocyclohexane (gamma-HCH or lindane) as it also catalyzes conversion of 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN) to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-DDOL) via the intermediate 2,4,5-trichloro-2,5-cyclohexadiene-1-ol (2,4,5-DNOL) (PubMed:7691794). This degradation pathway allows S.japonicum UT26 to grow on gamma-HCH as the sole source of carbon and energy.<ref>PMID:10100638</ref> <ref>PMID:7691794</ref> <ref>PMID:9293022</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mj5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mj5 ConSurf].
<div style="clear:both"></div>


===LINB (haloalkane dehalogenase) from sphingomonas paucimobilis UT26 at atomic resolution===
==See Also==
 
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
{{ABSTRACT_PUBMED_14744129}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[1mj5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJ5 OCA].
</StructureSection>
 
[[Category: Large Structures]]
==Reference==
<ref group="xtra">PMID:014744129</ref><ref group="xtra">PMID:018754631</ref><references group="xtra"/>
[[Category: Haloalkane dehalogenase]]
[[Category: Sphingomonas paucimobilis]]
[[Category: Sphingomonas paucimobilis]]
[[Category: Damborsky, J.]]
[[Category: Damborsky J]]
[[Category: Oakley, A J.]]
[[Category: Oakley AJ]]
[[Category: Wilce, M C.]]
[[Category: Wilce MC]]
[[Category: 4-cyclohexadiene dehalogenase]]
[[Category: 6-tetrachloro-1]]
[[Category: Alpha/beta-hydrolase]]
[[Category: Gamma-hexachlorocyclohexane degradation]]
[[Category: Haloalkane dehalogenase]]
[[Category: Hydrolase]]
[[Category: Linb]]
[[Category: Ultra high resolution]]

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