1j74: Difference between revisions

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-[[Image:1j74.png|left|200px]]
-{{STRUCTURE_1j74|  PDB=1j74  |  SCENE=  }}
+==Crystal Structure of Mms2==
+<StructureSection load='1j74' size='340' side='right'caption='[[1j74]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1j74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J74 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j74 OCA], [https://pdbe.org/1j74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j74 RCSB], [https://www.ebi.ac.uk/pdbsum/1j74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j74 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/UB2V2_HUMAN UB2V2_HUMAN] Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.<ref>PMID:9705497</ref> <ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:20061386</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/1j74_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j74 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The ubiquitin conjugating enzyme complex Mms2-Ubc13 plays a key role in post-replicative DNA repair in yeast and the NF-kappaB signal transduction pathway in humans. This complex assembles novel polyubiquitin chains onto yet uncharacterized protein targets. Here we report the crystal structure of a complex between hMms2 (Uev1) and hUbc13 at 1.85 A resolution and a structure of free hMms2 at 1.9 A resolution. These structures reveal that the hMms2 monomer undergoes a localized conformational change upon interaction with hUbc13. The nature of the interface provides a physical basis for the preference of Mms2 for Ubc13 as a partner over a variety of other structurally similar ubiquitin-conjugating enzymes. The structure of the hMms2-hUbc13 complex provides the conceptual foundation for understanding the mechanism of Lys 63 multiubiquitin chain assembly and for its interactions with the RING finger proteins Rad5 and Traf6.
-===Crystal Structure of Mms2===
+Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13.,Moraes TF, Edwards RA, McKenna S, Pastushok L, Xiao W, Glover JN, Ellison MJ Nat Struct Biol. 2001 Aug;8(8):669-73. PMID:11473255<ref>PMID:11473255</ref>
-{{ABSTRACT_PUBMED_11473255}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1j74" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[1j74]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J74 OCA].
+*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:011473255</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Edwards, R A.]]
+[[Category: Large Structures]]
-[[Category: Ellison, M J.]]
+[[Category: Edwards RA]]
-[[Category: Glover, J N.M.]]
+[[Category: Ellison MJ]]
-[[Category: McKenna, S.]]
+[[Category: Glover JNM]]
-[[Category: Moraes, T F.]]
+[[Category: McKenna S]]
-[[Category: Pastushok, L.]]
+[[Category: Moraes TF]]
-[[Category: Xiao, W.]]
+[[Category: Pastushok L]]
-[[Category: Dna repair]]
+[[Category: Xiao W]]
-[[Category: Mms2]]
-[[Category: Ubc]]
-[[Category: Ubiquitin]]
-[[Category: Uev]]
-[[Category: Unknown function]]