4hus: Difference between revisions

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-[[Image:4hus.jpg|left|200px]]
-{{STRUCTURE_4hus|  PDB=4hus  |  SCENE=  }}
+==Crystal structure of streptogramin group A antibiotic acetyltransferase VatA from Staphylococcus aureus in complex with virginiamycin M1==
+<StructureSection load='4hus' size='340' side='right'caption='[[4hus]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4hus]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HUS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.36&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=VIR:VIRGINIAMYCIN+M1'>VIR</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hus OCA], [https://pdbe.org/4hus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hus RCSB], [https://www.ebi.ac.uk/pdbsum/4hus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hus ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/VATA_STAAU VATA_STAAU] Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Combinations of streptogramins of group A and B (i.e. dalfoprisin and quinipristin) are "last-resort" antibiotics for treatment of infections caused by Gram-positive pathogens including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. Resistance to streptogramins has arisen via multiple mechanisms, including deactivation of the group A component by the large family of virginamycin O-acetyltransferase (Vat) enzymes. Despite the structural elucidation performed for the VatD acetyltransferase which provided a general molecular framework for activity, detailed characterization of the essential catalytic and antibiotic substrate-binding determinants in Vat enzymes is still lacking. We have determined the crystal structure of S. aureus VatA in apo, virginiamycin M1- and acetyl CoA-bound forms and provide an extensive mutagenesis and functional analysis of the structural determinants required for catalysis and streptogramin A recognition. Based on an updated genomic survey across the Vat enzyme family we identified key conserved residues critical for VatA activity that are not part of the O-acetylation catalytic apparatus. Exploiting such constraints of the Vat active site may lead to development of streptogramin A compounds that evade inactivation by Vat enzymes while retaining binding to their ribosomal target.
-===Crystal structure of streptogramin group A antibiotic acetyltransferase VatA from Staphylococcus aureus in complex with virginiamycin M1===
+Potential for reduction of streptogramin A resistance revealed by structural analysis of the VatA acetyltransferase.,Stogios PJ, Kuhn ML, Evdokimova E, Courvalin P, Anderson WF, Savchenko A Antimicrob Agents Chemother. 2014 Sep 15. pii: AAC.03743-14. PMID:25223995<ref>PMID:25223995</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-[[4hus]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HUS OCA].
+<div class="pdbe-citations 4hus" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Staphylococcus aureus]]
-[[Category: Anderson, W F.]]
+[[Category: Anderson WF]]
-[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
+[[Category: Courvalin P]]
-[[Category: Courvalin, P.]]
+[[Category: Di Leo R]]
-[[Category: Evdokimova, E.]]
+[[Category: Evdokimova E]]
-[[Category: Krishnamoorthy, M.]]
+[[Category: Krishnamoorthy M]]
-[[Category: Leo, R Di.]]
+[[Category: Minasov G]]
-[[Category: Minasov, G.]]
+[[Category: Savchenko A]]
-[[Category: Savchenko, A.]]
+[[Category: Stogios PJ]]
-[[Category: Stogios, P J.]]
+[[Category: Wawrzak Z]]
-[[Category: Wawrzak, Z.]]
+[[Category: Yim V]]
-[[Category: Yim, V.]]
-[[Category: Acetyl coenzyme some]]
-[[Category: Antibiotic resistance]]
-[[Category: Coenzyme some]]
-[[Category: Dalfopristin]]
-[[Category: Hexapeptide repeat acyltransferase]]
-[[Category: Intracellular]]
-[[Category: National institute of allergy and infectious disease]]
-[[Category: Niaid]]
-[[Category: Streptogramin some]]
-[[Category: Streptogramin group a antibiotic acetyltransferase]]
-[[Category: Streptogramin group a antibiotic]]
-[[Category: Structural genomic]]
-[[Category: Transferase-antibiotic complex]]
-[[Category: Virginiamycin m1]]