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[[Image:1qa6.gif|left|200px]]<br /><applet load="1qa6" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1qa6, resolution 2.800&Aring;" />
'''CRYSTAL STRUCTURE OF A CONSERVED RIBOSOMAL PROTEIN-RNA COMPLEX'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A CONSERVED RIBOSOMAL PROTEIN-RNA COMPLEX==
The structure of a highly conserved complex between a 58-nucleotide domain of large subunit ribosomal RNA and the RNA-binding domain of ribosomal protein L11 has been solved at 2.8 angstrom resolution. It reveals a precisely folded RNA structure that is stabilized by extensive tertiary contacts and contains an unusually large core of stacked bases. A bulge loop base from one hairpin of the RNA is intercalated into the distorted major groove of another helix; the protein locks this tertiary interaction into place by binding to the intercalated base from the minor groove side. This direct interaction with a key ribosomal RNA tertiary interaction suggests that part of the role of L11 is to stabilize an unusual RNA fold within the ribosome.
<StructureSection load='1qa6' size='340' side='right'caption='[[1qa6]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1qa6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QA6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OS:OSMIUM+ION'>OS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qa6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qa6 OCA], [https://pdbe.org/1qa6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qa6 RCSB], [https://www.ebi.ac.uk/pdbsum/1qa6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qa6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL11_GEOSE RL11_GEOSE] Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qa/1qa6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qa6 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1QA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=OS:'>OS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QA6 OCA].
*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of a conserved ribosomal protein-RNA complex., Conn GL, Draper DE, Lattman EE, Gittis AG, Science. 1999 May 14;284(5417):1171-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10325228 10325228]
[[Category: Escherichia coli]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Conn, G L.]]
[[Category: Conn GL]]
[[Category: Draper, D E.]]
[[Category: Draper DE]]
[[Category: Gittis, A G.]]
[[Category: Gittis AG]]
[[Category: Lattman, E E.]]
[[Category: Lattman EE]]
[[Category: MG]]
[[Category: OS]]
[[Category: antibiotic binding]]
[[Category: e rna-protein interaction]]
[[Category: minor groove binding]]
[[Category: ribosomal rna]]
[[Category: tertiary structur]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:29 2008''

Latest revision as of 11:14, 14 February 2024

CRYSTAL STRUCTURE OF A CONSERVED RIBOSOMAL PROTEIN-RNA COMPLEXCRYSTAL STRUCTURE OF A CONSERVED RIBOSOMAL PROTEIN-RNA COMPLEX

Structural highlights

1qa6 is a 4 chain structure with sequence from Escherichia coli and Geobacillus stearothermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL11_GEOSE Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1qa6, resolution 2.80Å

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