4hip: Difference between revisions

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-'''Unreleased structure'''
-The entry 4hip is ON HOLD
+==Crystal structure of the Pseudomonas aeruginosa azurin, H126NO YOH109==
+<StructureSection load='4hip' size='340' side='right'caption='[[4hip]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4hip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HIP FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NIY:META-NITRO-TYROSINE'>NIY</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hip OCA], [https://pdbe.org/4hip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hip RCSB], [https://www.ebi.ac.uk/pdbsum/4hip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hip ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have designed ruthenium-modified Pseudomonas aeruginosa azurins that incorporate 3-nitrotyrosine (NO2YOH) between Ru(2,2'-bipyridine)2(imidazole)(histidine) and Cu redox centers in electron transfer (ET) pathways. We investigated the structures and reactivities of three different systems: RuH107NO2YOH109, RuH124NO2YOH122, and RuH126NO2YOH122. RuH107NO2YOH109, unlabeled H124NO2YOH122, and unlabeled H126NO2YOH122 were structurally characterized. The pKa's of NO2YOH at positions 122 and 109 are 7.2 and 6.0, respectively. Reduction potentials of 3-nitrotyrosinate (NO2YO-)-modified azurins were estimated from cyclic and differential pulse voltammetry data: oxidation of NO2YO-122 occurs near 1.1 versus NHE; oxidation of NO2YO-109 is near 1.2 V. Our analysis of transient optical spectroscopic experiments indicates that hopping via NO2YO- enhances CuI oxidation rates over single-step ET by factors of 32 (RuH107NO2YO-109), 46 (RuH126NO2YO-122), and 13 (RuH124NO2YO-122).
-Authors: Herrera, N., Warren, J.J., Gray, H.B.
+Electron Flow through Nitrotyrosinate in Pseudomonas aeruginosa Azurin.,Warren JJ, Herrera N, Hill MG, Winkler JR, Gray HB J Am Chem Soc. 2013 Jul 16. PMID:23859602<ref>PMID:23859602</ref>
-Description: Crystal structure of the Pseudomonas aeruginosa azurin, H126NO YOH109
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4hip" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Azurin 3D structures|Azurin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Pseudomonas aeruginosa PAO1]]
+[[Category: Gray HB]]
+[[Category: Herrera N]]
+[[Category: Warren JJ]]