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[[Image:1plb.gif|left|200px]]<br /><applet load="1plb" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1plb" />
'''HIGH-RESOLUTION SOLUTION STRUCTURE OF REDUCED PARSLEY PLASTOCYANIN'''<br />


==Overview==
==HIGH-RESOLUTION SOLUTION STRUCTURE OF REDUCED PARSLEY PLASTOCYANIN==
A high-resolution three-dimensional solution structure of parsley plastocyanin has been determined using 1H-NMR-derived data. An ensemble of 30 conformers has been calculated, exhibiting an atomic root mean square distribution about the mean coordinate positions of 0.37 +/- 0.03 A for backbone atoms and 0.75 +/- 0.04 A for all heavy atoms. (These values exclude residues 8-10 which are disordered.) The global fold of parsley plastocyanin is closely similar to those of other plastocyanins which have been structurally characterized by X-ray diffraction and NMR methods. However, deletion of residues at positions 57 and 58 of the consensus plastocyanin sequence causes elimination of a turn found in most higher plant plastocyanins. This turn is located in an acidic patch binding site, which consists of two clusters of acidic residues at positions 42-45 and 59-61. These residues surround the side chain of Tyr 83, which has been shown to be involved in binding of and electron transfer from cytochrome f, one of plastocyanin's physiological partners. The acidic recognition site is further disrupted in parsley plastocyanin by nonconservative substitution of two charged residues at positions 59 and 60. The NMR-derived structures show that E53, E85, and E95 compensate for these substitutions and give parsley plastocyanin an acidic recognition site of similar extent to that of other higher plant plastocyanins.(ABSTRACT TRUNCATED AT 250 WORDS)
<StructureSection load='1plb' size='340' side='right'caption='[[1plb]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1plb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PLB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1plb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1plb OCA], [https://pdbe.org/1plb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1plb RCSB], [https://www.ebi.ac.uk/pdbsum/1plb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1plb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PLAS_PETCR PLAS_PETCR] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pl/1plb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1plb ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PLB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLB OCA].
*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
High-resolution solution structure of reduced parsley plastocyanin., Bagby S, Driscoll PC, Harvey TS, Hill HA, Biochemistry. 1994 May 31;33(21):6611-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8204598 8204598]
[[Category: Large Structures]]
[[Category: Petroselinum crispum]]
[[Category: Petroselinum crispum]]
[[Category: Single protein]]
[[Category: Bagby S]]
[[Category: Bagby, S.]]
[[Category: Driscoll PC]]
[[Category: Driscoll, P C.]]
[[Category: Harvey TS]]
[[Category: Harvey, T S.]]
[[Category: Hill HAO]]
[[Category: Hill, H A.O.]]
[[Category: CU]]
[[Category: electron transport protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:02 2008''

Latest revision as of 08:56, 17 April 2024

HIGH-RESOLUTION SOLUTION STRUCTURE OF REDUCED PARSLEY PLASTOCYANINHIGH-RESOLUTION SOLUTION STRUCTURE OF REDUCED PARSLEY PLASTOCYANIN

Structural highlights

1plb is a 1 chain structure with sequence from Petroselinum crispum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PLAS_PETCR Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

Drag the structure with the mouse to rotate

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