2yoe: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2yoe.jpg|left|200px]]
-{{STRUCTURE_2yoe|  PDB=2yoe  |  SCENE=  }}
+==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with GABA and flurazepam==
+<StructureSection load='2yoe' size='340' side='right'caption='[[2yoe]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2yoe]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4a96 4a96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YOE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABU:GAMMA-AMINO-BUTANOIC+ACID'>ABU</scene>, <scene name='pdbligand=FL7:FLURAZEPAM'>FL7</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yoe OCA], [https://pdbe.org/2yoe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yoe RCSB], [https://www.ebi.ac.uk/pdbsum/2yoe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yoe ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ELIC_DICCH ELIC_DICCH]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+GABA(A) receptors are pentameric ligand-gated ion channels involved in fast inhibitory neurotransmission and are allosterically modulated by the anxiolytic, anticonvulsant, and sedative-hypnotic benzodiazepines. Here we show that the prokaryotic homolog ELIC also is activated by GABA and is modulated by benzodiazepines with effects comparable to those at GABA(A) receptors. Crystal structures reveal important features of GABA recognition and indicate that benzodiazepines, depending on their concentration, occupy two possible sites in ELIC. An intrasubunit site is adjacent to the GABA-recognition site but faces the channel vestibule. A second intersubunit site partially overlaps with the GABA site and likely corresponds to a low-affinity benzodiazepine-binding site in GABA(A) receptors that mediates inhibitory effects of the benzodiazepine flurazepam. Our study offers a structural view how GABA and benzodiazepines are recognized at a GABA-activated ion channel.
-===X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with GABA and flurazepam===
+Pentameric ligand-gated ion channel ELIC is activated by GABA and modulated by benzodiazepines.,Spurny R, Ramerstorfer J, Price K, Brams M, Ernst M, Nury H, Verheij M, Legrand P, Bertrand D, Bertrand S, Dougherty DA, de Esch IJ, Corringer PJ, Sieghart W, Lummis SC, Ulens C Proc Natl Acad Sci U S A. 2012 Oct 3. PMID:23035248<ref>PMID:23035248</ref>
-{{ABSTRACT_PUBMED_23035248}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2yoe" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[2yoe]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4a96 4a96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YOE OCA].
+*[[Ion channels 3D structures|Ion channels 3D structures]]
-[[Category: Erwinia chrysanthemi]]
+== References ==
-[[Category: Brams, M.]]
+<references/>
-[[Category: Legrand, P.]]
+__TOC__
-[[Category: Nury, H.]]
+</StructureSection>
-[[Category: Spurny, R.]]
+[[Category: Dickeya chrysanthemi]]
-[[Category: Ulens, C.]]
+[[Category: Large Structures]]
-[[Category: Benzodiazepine]]
+[[Category: Brams M]]
-[[Category: Cys-loop receptor]]
+[[Category: Legrand P]]
-[[Category: Gaba-a receptor]]
+[[Category: Nury H]]
-[[Category: Transport protein]]
+[[Category: Spurny R]]
+[[Category: Ulens C]]