1ox9: Difference between revisions

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-[[Image:1ox9.gif|left|200px]]<br /><applet load="1ox9" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1ox9, resolution 2.9&Aring;" />
-'''Crystal structure of SspB-ssrA complex'''<br />
-==Overview==
+==Crystal structure of SspB-ssrA complex==
-In prokaryotes, incomplete or misfolded polypeptides emanating from a stalled ribosome are marked for degradation by the addition of an 11 residue peptide (AANDENYALAA) to their C terminus. Substrates containing this conserved degradation signal, the SsrA tag, are targeted to specific proteases including ClpXP and ClpAP. SspB was originally characterized as a stringent starvation protein and has been found to bind specifically to SsrA-tagged proteins and to enhance recognition of these proteins by the ClpXP degradation machine. Here, we report the crystal structures of SspB alone and in complex with an SsrA peptide. Unexpectedly, SspB exhibits a fold found in Sm-family RNA binding proteins. The dimeric SspB structures explain the key determinants for recognition of the SsrA tag and define a hydrophobic channel that may bind unfolded substrates.
+<StructureSection load='1ox9' size='340' side='right'caption='[[1ox9]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ox9]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OX9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ox9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ox9 OCA], [https://pdbe.org/1ox9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ox9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ox9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ox9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SSPB_ECOLI SSPB_ECOLI] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth.<ref>PMID:11009422</ref> <ref>PMID:15371343</ref> <ref>PMID:12887894</ref>   Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion.<ref>PMID:11009422</ref> <ref>PMID:15371343</ref> <ref>PMID:12887894</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1ox9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ox9 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-OX9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o127:h6 Escherichia coli o127:h6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX9 OCA].
+*[[Stringent starvation protein 3D structures|Stringent starvation protein 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine., Song HK, Eck MJ, Mol Cell. 2003 Jul;12(1):75-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12887894 12887894]
+__TOC__
-[[Category: Escherichia coli o127:h6]]
+</StructureSection>
-[[Category: Single protein]]
+[[Category: Escherichia coli O157:H7]]
-[[Category: Eck, M J.]]
+[[Category: Large Structures]]
-[[Category: Song, H K.]]
+[[Category: Eck MJ]]
-[[Category: sspb-ssra]]
+[[Category: Song HK]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:41 2008''