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[[Image:1ct9.png|left|200px]]


{{STRUCTURE_1ct9|  PDB=1ct9  |  SCENE=  }}
==CRYSTAL STRUCTURE OF ASPARAGINE SYNTHETASE B FROM ESCHERICHIA COLI==
 
<StructureSection load='1ct9' size='340' side='right'caption='[[1ct9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
===CRYSTAL STRUCTURE OF ASPARAGINE SYNTHETASE B FROM ESCHERICHIA COLI===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[1ct9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CT9 FirstGlance]. <br>
{{ABSTRACT_PUBMED_10587437}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ct9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct9 OCA], [https://pdbe.org/1ct9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ct9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ct9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ct9 ProSAT]</span></td></tr>
[[1ct9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT9 OCA].  
</table>
 
== Function ==
==Reference==
[https://www.uniprot.org/uniprot/ASNB_ECOLI ASNB_ECOLI] Catalyzes the ATP-dependent conversion of aspartate into asparagine, using glutamine as a source of nitrogen. Can also use ammonia as the nitrogen source in vitro, albeit with lower efficiency. As nucleotide substrates, ATP and dATP are utilized at a similar rate in both the glutamine- and ammonia-dependent reactions, whereas GTP utilization is only 15% that of ATP, and CTP, UTP, ITP and XTP are very poor or not substrates. Also exhibits glutaminase activity.<ref>PMID:7907328</ref> <ref>PMID:6102982</ref> <ref>PMID:20853825</ref>
<ref group="xtra">PMID:010587437</ref><references group="xtra"/>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/1ct9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ct9 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Boehlein, S K.]]
[[Category: Large Structures]]
[[Category: Holden, H M.]]
[[Category: Boehlein SK]]
[[Category: Larsen, T M.]]
[[Category: Holden HM]]
[[Category: Rayment, I.]]
[[Category: Larsen TM]]
[[Category: Richards, N G.J.]]
[[Category: Rayment I]]
[[Category: Schuster, S M.]]
[[Category: Richards NGJ]]
[[Category: Thoden, J B.]]
[[Category: Schuster SM]]
[[Category: Amidotransferase]]
[[Category: Thoden JB]]
[[Category: Asparagine biosynthesis]]
[[Category: Ligase]]
[[Category: Substrate channeling]]

Latest revision as of 09:45, 7 February 2024

CRYSTAL STRUCTURE OF ASPARAGINE SYNTHETASE B FROM ESCHERICHIA COLICRYSTAL STRUCTURE OF ASPARAGINE SYNTHETASE B FROM ESCHERICHIA COLI

Structural highlights

1ct9 is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ASNB_ECOLI Catalyzes the ATP-dependent conversion of aspartate into asparagine, using glutamine as a source of nitrogen. Can also use ammonia as the nitrogen source in vitro, albeit with lower efficiency. As nucleotide substrates, ATP and dATP are utilized at a similar rate in both the glutamine- and ammonia-dependent reactions, whereas GTP utilization is only 15% that of ATP, and CTP, UTP, ITP and XTP are very poor or not substrates. Also exhibits glutaminase activity.[1] [2] [3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Boehlein SK, Richards NG, Schuster SM. Glutamine-dependent nitrogen transfer in Escherichia coli asparagine synthetase B. Searching for the catalytic triad. J Biol Chem. 1994 Mar 11;269(10):7450-7. PMID:7907328
  2. ↑ Humbert R, Simoni RD. Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli. J Bacteriol. 1980 Apr;142(1):212-20. PMID:6102982
  3. ↑ Meyer ME, Gutierrez JA, Raushel FM, Richards NG. A conserved glutamate controls the commitment to acyl-adenylate formation in asparagine synthetase. Biochemistry. 2010 Nov 2;49(43):9391-401. doi: 10.1021/bi1010688. PMID:20853825 doi:http://dx.doi.org/10.1021/bi1010688

1ct9, resolution 2.00Å

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