1eg0: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
No edit summary
No edit summary
 
(9 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1eg0.png|left|200px]]


{{STRUCTURE_1eg0|  PDB=1eg0  |  SCENE=  }}
==FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME==
 
<SX load='1eg0' size='340' side='right' viewer='molstar' caption='[[1eg0]], [[Resolution|resolution]] 11.50&Aring;' scene=''>
===FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[1eg0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EG0 FirstGlance]. <br>
{{ABSTRACT_PUBMED_10721991}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg0 OCA], [https://pdbe.org/1eg0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eg0 RCSB], [https://www.ebi.ac.uk/pdbsum/1eg0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eg0 ProSAT]</span></td></tr>
[[1eg0]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG0 OCA].  
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS4_BACSU RS4_BACSU] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.  With S5 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations).  S4 represses its own expression; it is not know if this is at the level of translation or of mRNA stability.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1eg0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eg0 ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Ribosomal protein L11|Ribosomal protein L11]]
*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
*[[Ribosomal protein L6|Ribosomal protein L6]]
*[[Ribosomal protein L6|Ribosomal protein L6]]
*[[Ribosomal protein S15|Ribosomal protein S15]]
__TOC__
*[[Ribosomal protein S17|Ribosomal protein S17]]
</SX>
*[[Ribosomal protein S20|Ribosomal protein S20]]
 
==Reference==
<ref group="xtra">PMID:010721991</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Agrawal, R K.]]
[[Category: Large Structures]]
[[Category: Frank, J.]]
[[Category: Agrawal RK]]
[[Category: Gabashvili, I S.]]
[[Category: Frank J]]
[[Category: Grassucci, R A.]]
[[Category: Gabashvili IS]]
[[Category: Penczek, P.]]
[[Category: Grassucci RA]]
[[Category: Spahn, C M.T.]]
[[Category: Penczek P]]
[[Category: Svergun, D I.]]
[[Category: Spahn CMT]]
[[Category: 70s ribosome]]
[[Category: Svergun DI]]
[[Category: Low resolution model]]
[[Category: Ribosome]]

Latest revision as of 10:02, 7 February 2024

FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOMEFITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME

1eg0, resolution 11.50Å

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA