3b07: Difference between revisions

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-[[Image:3b07.png|left|200px]]
-{{STRUCTURE_3b07|  PDB=3b07  |  SCENE=  }}
+==Crystal structure of octameric pore form of gamma-hemolysin from Staphylococcus aureus==
+<StructureSection load='3b07' size='340' side='right'caption='[[3b07]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3b07]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B07 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.495&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b07 OCA], [https://pdbe.org/3b07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b07 RCSB], [https://www.ebi.ac.uk/pdbsum/3b07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b07 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0H3JX61_STAAM A0A0H3JX61_STAAM]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Staphylococcal gamma-hemolysin is a bicomponent pore-forming toxin composed of LukF and Hlg2. These proteins are expressed as water-soluble monomers and then assemble into the oligomeric pore form on the target cell. Here, we report the crystal structure of the octameric pore form of gamma-hemolysin at 2.5 A resolution, which is the first high-resolution structure of a beta-barrel transmembrane protein composed of two proteins reported to date. The octameric assembly consists of four molecules of LukF and Hlg2 located alternately in a circular pattern, which explains the biochemical data accumulated over the past two decades. The structure, in combination with the monomeric forms, demonstrates the elaborate molecular machinery involved in pore formation by two different molecules, in which interprotomer electrostatic interactions using loops connecting beta2 and beta3 (loop A: Asp43-Lys48 of LukF and Lys37-Lys43 of Hlg2) play pivotal roles as the structural determinants for assembly through unwinding of the N-terminal beta-strands (amino-latch) of the adjacent protomer, releasing the transmembrane stem domain folded into a beta-sheet in the monomer (prestem), and interaction with the adjacent protomer.
-===Crystal structure of octameric pore form of gamma-hemolysin from Staphylococcus aureus===
+Crystal structure of the octameric pore of staphylococcal gamma-hemolysin reveals the beta-barrel pore formation mechanism by two components.,Yamashita K, Kawai Y, Tanaka Y, Hirano N, Kaneko J, Tomita N, Ohta M, Kamio Y, Yao M, Tanaka I Proc Natl Acad Sci U S A. 2011 Oct 18;108(42):17314-9. Epub 2011 Oct 3. PMID:21969538<ref>PMID:21969538</ref>
-{{ABSTRACT_PUBMED_21969538}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3b07" style="background-color:#fffaf0;"></div>
-[[3b07]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B07 OCA].
 ==See Also==
-*[[Hemolysin|Hemolysin]]
+*[[Hemolysin 3D structures|Hemolysin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021969538</ref><references group="xtra"/>
+__TOC__
-[[Category: Staphylococcus aureus]]
+</StructureSection>
-[[Category: Kawai, Y.]]
+[[Category: Large Structures]]
-[[Category: Tanaka, I.]]
+[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-[[Category: Tanaka, Y.]]
+[[Category: Kawai Y]]
-[[Category: Yamashita, K.]]
+[[Category: Tanaka I]]
-[[Category: Yao, M.]]
+[[Category: Tanaka Y]]
-[[Category: Protein complex]]
+[[Category: Yamashita K]]
-[[Category: Toxin]]
+[[Category: Yao M]]