2xgi: Difference between revisions

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-[[Image:2xgi.png|left|200px]]
-{{STRUCTURE_2xgi|  PDB=2xgi  |  SCENE=  }}
+==Crystal structure of Barley Beta-Amylase complexed with 3,4- epoxybutyl alpha-D-glucopyranoside==
+<StructureSection load='2xgi' size='340' side='right'caption='[[2xgi]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2xgi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGI FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EBQ:2-[(2R)-OXIRAN-2-YL]ETHYL+ALPHA-D-GLUCOPYRANOSIDE'>EBQ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J5B:(3R)-3-hydroxybutyl+alpha-D-glucopyranoside'>J5B</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xgi OCA], [https://pdbe.org/2xgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xgi RCSB], [https://www.ebi.ac.uk/pdbsum/2xgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xgi ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AMYB_HORVU AMYB_HORVU]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in germinating barley grains. As a precursor to in vivo studies, here we report systematic analysis of the reversible and irreversible inhibition of the major beta-amylase of the grain endosperm (BMY1). The molecular basis of inhibitor action was defined through high resolution X-ray crystallography studies of unliganded barley beta-amylase, as well as its complexes with glycone site binder disaccharide iminosugar G1M, irreversible inhibitors alpha-epoxypropyl and alpha-epoxybutyl glucosides, which target the enzyme's catalytic residues, and the aglycone site binders acarbose and alpha-cyclodextrin.
-===Crystal structure of Barley Beta-Amylase complexed with 3,4- epoxybutyl alpha-D-glucopyranoside===
+Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley beta-amylase.,Rejzek M, Stevenson CE, Southard AM, Stanley D, Denyer K, Smith AM, Naldrett MJ, Lawson DM, Field RA Mol Biosyst. 2010 Nov 18. PMID:21085740<ref>PMID:21085740</ref>
-{{ABSTRACT_PUBMED_21085740}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2xgi" style="background-color:#fffaf0;"></div>
-[[2xgi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGI OCA].
 ==See Also==
-*[[Alpha-Amylase|Alpha-Amylase]]
+*[[Amylase 3D structures|Amylase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021085740</ref><references group="xtra"/>
+__TOC__
-[[Category: Beta-amylase]]
+</StructureSection>
 [[Category: Hordeum vulgare]]
-[[Category: Denyer, K.]]
+[[Category: Large Structures]]
-[[Category: Field, R A.]]
+[[Category: Denyer K]]
-[[Category: Lawson, D M.]]
+[[Category: Field RA]]
-[[Category: Naldrett, M J.]]
+[[Category: Lawson DM]]
-[[Category: Rejzek, M.]]
+[[Category: Naldrett MJ]]
-[[Category: Smith, A M.]]
+[[Category: Rejzek M]]
-[[Category: Southard, A M.]]
+[[Category: Smith AM]]
-[[Category: Stanley, D.]]
+[[Category: Southard AM]]
-[[Category: Stevenson, C E.M.]]
+[[Category: Stanley D]]
-[[Category: Carbohydrate metabolism]]
+[[Category: Stevenson CEM]]
-[[Category: Germination]]
-[[Category: Glycosidase]]
-[[Category: Glycosyl hydrolase family 14]]
-[[Category: Hydrolase]]
-[[Category: Starch degradation]]