4aab: Difference between revisions

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-[[Image:4aab.png|left|200px]]
-{{STRUCTURE_4aab|  PDB=4aab  |  SCENE=  }}
+==Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3')==
+<StructureSection load='4aab' size='340' side='right'caption='[[4aab]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4aab]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AAB FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aab OCA], [https://pdbe.org/4aab PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aab RCSB], [https://www.ebi.ac.uk/pdbsum/4aab PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aab ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Homing endonucleases represent protein scaffolds that provide powerful tools for genome manipulation, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. The basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. Protein-DNA interaction engineering of homing endonucleases has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Protein-DNA interface studies have been focused mostly on specific contacts between amino acid side chains and bases to redesign the binding interface. However, it has been shown that 4 bp in the central DNA sequence of the 22-bp substrate of a homing endonuclease (I-CreI), which do not show specific protein-DNA interactions, is not devoid of content information. Here, we analyze the mechanism of target discrimination in this substrate region by the I-CreI protein, determining how it can occur independently of the specific protein-DNA interactions. Our data suggest the important role of indirect readout in this substrate region, opening the possibility for a fully rational search of new target sequences, thus improving the development of redesigned enzymes for therapeutic and biotechnological applications.
-===Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3')===
+Non-specific protein-DNA interactions control I-CreI target binding and cleavage.,Molina R, Redondo P, Stella S, Marenchino M, D'Abramo M, Gervasio FL, Charles Epinat J, Valton J, Grizot S, Duchateau P, Prieto J, Montoya G Nucleic Acids Res. 2012 Apr 11. PMID:22495931<ref>PMID:22495931</ref>
-{{ABSTRACT_PUBMED_22495931}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 4aab" style="background-color:#fffaf0;"></div>
-[[4aab]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAB OCA].
 ==See Also==
-*[[Endonuclease|Endonuclease]]
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:022495931</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Chlamydomonas reinhardtii]]
-[[Category: Abramo, M D.]]
+[[Category: Large Structures]]
-[[Category: Duchateau, P.]]
+[[Category: Synthetic construct]]
-[[Category: Epinat, J C.]]
+[[Category: D'Abramo M]]
-[[Category: Gervasio, F L.]]
+[[Category: Duchateau P]]
-[[Category: Grizot, S.]]
+[[Category: Epinat JC]]
-[[Category: Marenchino, M.]]
+[[Category: Gervasio FL]]
-[[Category: Molina, R.]]
+[[Category: Grizot S]]
-[[Category: Montoya, G.]]
+[[Category: Marenchino M]]
-[[Category: Prieto, J.]]
+[[Category: Molina R]]
-[[Category: Redondo, P.]]
+[[Category: Montoya G]]
-[[Category: Stella, S.]]
+[[Category: Prieto J]]
-[[Category: Valton, J.]]
+[[Category: Redondo P]]
-[[Category: Gene targeting]]
+[[Category: Stella S]]
-[[Category: Homing endonuclease]]
+[[Category: Valton J]]
-[[Category: Hydrolase-dna complex]]
-[[Category: Protein-dna interaction]]