1l2t: Difference between revisions

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-[[Image:1l2t.gif|left|200px]]<br /><applet load="1l2t" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1l2t, resolution 1.90&Aring;" />
-'''Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette'''<br />
-==Overview==
+==Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette==
-It has been proposed that the reaction cycle of ATP binding cassette (ABC) transporters is driven by dimerization of their ABC motor domains upon binding ATP at their mutual interface. However, no such ATP sandwich complex has been observed for an ABC from an ABC transporter. In this paper, we report the crystal structure of a stable dimer formed by the E171Q mutant of the MJ0796 ABC, which is hydrolytically inactive due to mutation of the catalytic base. The structure shows a symmetrical dimer in which two ATP molecules are each sandwiched between the Walker A motif in one subunit and the LSGGQ signature motif in the other subunit. These results establish the stereochemical basis of the power stroke of ABC transporter pumps.
+<StructureSection load='1l2t' size='340' side='right'caption='[[1l2t]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1l2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2T FirstGlance]. <br>
-L2T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=ATP:'>ATP</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2T OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2t OCA], [https://pdbe.org/1l2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2t RCSB], [https://www.ebi.ac.uk/pdbsum/1l2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2t ProSAT]</span></td></tr>
-ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer., Smith PC, Karpowich N, Millen L, Moody JE, Rosen J, Thomas PJ, Hunt JF, Mol Cell. 2002 Jul;10(1):139-49. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12150914 12150914]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Y796_METJA Y796_METJA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/1l2t_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l2t ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Methanocaldococcus jannaschii]]
-[[Category: Single protein]]
+[[Category: Hunt JF]]
-[[Category: Hunt, J F.]]
+[[Category: Karpowich N]]
-[[Category: Karpowich, N.]]
+[[Category: Rosen J]]
-[[Category: Rosen, J.]]
+[[Category: Smith PC]]
-[[Category: Smith, P C.]]
-[[Category: ATP]]
-[[Category: IPA]]
-[[Category: NA]]
-[[Category: abc transporters]]
-[[Category: atpase]]
-[[Category: nbd]]
-[[Category: walker-a]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:40 2008''