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[[Image:1k8k.gif|left|200px]]<br /><applet load="1k8k" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1k8k, resolution 2.0&Aring;" />
'''Crystal Structure of Arp2/3 Complex'''<br />


==Overview==
==Crystal Structure of Arp2/3 Complex==
We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are folded like actin, with distinctive surface features. Subunits ARPC2 p34 and ARPC4 p20 in the core of the complex associate through long carboxyl-terminal alpha helices and have similarly folded amino-terminal alpha/beta domains. ARPC1 p40 is a seven-blade beta propeller with an insertion that may associate with the side of an actin filament. ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament.
<StructureSection load='1k8k' size='340' side='right'caption='[[1k8k]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1k8k]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K8K FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k8k OCA], [https://pdbe.org/1k8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k8k RCSB], [https://www.ebi.ac.uk/pdbsum/1k8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k8k ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARP3_BOVIN ARP3_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/1k8k_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k8k ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1K8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8K OCA].
*[[Actin-related protein 3D structures|Actin-related protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of Arp2/3 complex., Robinson RC, Turbedsky K, Kaiser DA, Marchand JB, Higgs HN, Choe S, Pollard TD, Science. 2001 Nov 23;294(5547):1679-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11721045 11721045]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Choe, S.]]
[[Category: Choe S]]
[[Category: Higgs, H N.]]
[[Category: Higgs HN]]
[[Category: Kaiser, D A.]]
[[Category: Kaiser DA]]
[[Category: Marchand, J B.]]
[[Category: Marchand J-B]]
[[Category: Pollard, T D.]]
[[Category: Pollard TD]]
[[Category: Robinson, R C.]]
[[Category: Robinson RC]]
[[Category: Turbedsky, K.]]
[[Category: Turbedsky K]]
[[Category: beta-propeller]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:15 2008''

Latest revision as of 10:45, 7 February 2024

Crystal Structure of Arp2/3 ComplexCrystal Structure of Arp2/3 Complex

Structural highlights

1k8k is a 7 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ARP3_BOVIN Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1k8k, resolution 2.00Å

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