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[[Image:1k3e.jpg|left|200px]]<br /><applet load="1k3e" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1k3e, resolution 2.8&Aring;" />
'''Type III secretion chaperone CesT'''<br />


==Overview==
==Type III secretion chaperone CesT==
Several Gram-negative bacterial pathogens have evolved a type III secretion system to deliver virulence effector proteins directly into eukaryotic cells, a process essential for disease. This specialized secretion process requires customized chaperones specific for particular effector proteins. The crystal structures of the enterohemorrhagic Escherichia coli O157:H7 Tir-specific chaperone CesT and the Salmonella enterica SigD-specific chaperone SigE reveal a common overall fold and formation of homodimers. Site-directed mutagenesis suggests that variable, delocalized hydrophobic surfaces observed on the chaperone homodimers are responsible for specific binding to a particular effector protein. Isothermal titration calorimetry studies of Tir-CesT and enzymatic activity profiles of SigD-SigE indicate that the effector proteins are not globally unfolded in the presence of their cognate chaperones.
<StructureSection load='1k3e' size='340' side='right'caption='[[1k3e]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1k3e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K3E FirstGlance]. <br>
1K3E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3E OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3e OCA], [https://pdbe.org/1k3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k3e RCSB], [https://www.ebi.ac.uk/pdbsum/1k3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k3e ProSAT]</span></td></tr>
==Reference==
</table>
Structural and biochemical characterization of the type III secretion chaperones CesT and SigE., Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC, Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11685226 11685226]
== Function ==
[[Category: Escherichia coli]]
[https://www.uniprot.org/uniprot/CEST_ECO57 CEST_ECO57] Chaperone for the type III secretion of Tir. Probably stabilizes the protein, prevents inappropriate protein-proteininteractions and aids in secretion (By similarity).
[[Category: Single protein]]
__TOC__
[[Category: Bertero, M.]]
</StructureSection>
[[Category: Creagh, L.]]
[[Category: Escherichia coli O157:H7]]
[[Category: Finlay, B B.]]
[[Category: Large Structures]]
[[Category: Frey, E A.]]
[[Category: Bertero M]]
[[Category: Lim, D.]]
[[Category: Creagh L]]
[[Category: Luo, Y.]]
[[Category: Finlay BB]]
[[Category: Marcus, S L.]]
[[Category: Frey EA]]
[[Category: Pfuetzner, R A.]]
[[Category: Lim D]]
[[Category: Strynadka, N C.J.]]
[[Category: Luo Y]]
[[Category: Wenk, M R.]]
[[Category: Marcus SL]]
[[Category: chaperone]]
[[Category: Pfuetzner RA]]
[[Category: intimin receptor]]
[[Category: Strynadka NCJ]]
[[Category: secretion]]
[[Category: Wenk MR]]
[[Category: type iii]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:41 2008''

Latest revision as of 10:44, 7 February 2024

Type III secretion chaperone CesTType III secretion chaperone CesT

Structural highlights

1k3e is a 2 chain structure with sequence from Escherichia coli O157:H7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CEST_ECO57 Chaperone for the type III secretion of Tir. Probably stabilizes the protein, prevents inappropriate protein-proteininteractions and aids in secretion (By similarity).

1k3e, resolution 2.80Å

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