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[[Image:1k24.jpg|left|200px]]<br /><applet load="1k24" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1k24, resolution 2.03&Aring;" />
'''Crystal Structure of the OpcA Outer Membrane Adhesin/Invasin from Neisseria meningitidis'''<br />


==Overview==
==Crystal Structure of the OpcA Outer Membrane Adhesin/Invasin from Neisseria meningitidis==
OpcA is an integral outer membrane protein from Neisseria meningitidis, the causative agent of meningococcal meningitis and septicemia. It mediates the adhesion of N. meningitidis to epithelial and endothelial cells by binding to vitronectin and proteoglycan cell-surface receptors. Here, we report the determination of the crystal structure of OpcA to 2.0 A resolution. OpcA adopts a 10-stranded beta-barrel structure with extensive loop regions that protrude above the predicted surface of the membrane. The second external loop adopts an unusual conformation, traversing the axis of the beta-barrel and apparently blocking formation of a pore through the membrane. Loops 2, 3, 4, and 5 associate to form one side of a crevice in the external surface of the structure, the other side being formed by loop 1. The crevice is lined by positively charged residues and would form an ideal binding site for proteoglycan polysaccharide. The structure, therefore, suggests a model for how adhesion of this important human pathogen to proteoglycan is mediated at the molecular level.
<StructureSection load='1k24' size='340' side='right'caption='[[1k24]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1k24]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K24 FirstGlance]. <br>
1K24 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=1PE:'>1PE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K24 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k24 OCA], [https://pdbe.org/1k24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k24 RCSB], [https://www.ebi.ac.uk/pdbsum/1k24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k24 ProSAT]</span></td></tr>
Crystal structure of the OpcA integral membrane adhesin from Neisseria meningitidis., Prince SM, Achtman M, Derrick JP, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3417-21. Epub 2002 Mar 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11891340 11891340]
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q51227_NEIME Q51227_NEIME]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Neisseria meningitidis]]
[[Category: Neisseria meningitidis]]
[[Category: Single protein]]
[[Category: Achtman M]]
[[Category: Achtman, M.]]
[[Category: Derrick JP]]
[[Category: Derrick, J P.]]
[[Category: Prince SM]]
[[Category: Prince, S M.]]
[[Category: 1PE]]
[[Category: ZN]]
[[Category: adhesin]]
[[Category: beta barrel]]
[[Category: invasin]]
[[Category: membrane protein]]
[[Category: outer membrane]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:15 2008''

Latest revision as of 10:43, 7 February 2024

Crystal Structure of the OpcA Outer Membrane Adhesin/Invasin from Neisseria meningitidisCrystal Structure of the OpcA Outer Membrane Adhesin/Invasin from Neisseria meningitidis

Structural highlights

1k24 is a 1 chain structure with sequence from Neisseria meningitidis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.03Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q51227_NEIME

1k24, resolution 2.03Å

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