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[[Image:1jot.gif|left|200px]]<br /><applet load="1jot" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1jot, resolution 2.20&Aring;" />
'''STRUCTURE OF THE LECTIN MPA COMPLEXED WITH T-ANTIGEN DISACCHARIDE'''<br />


==Overview==
==STRUCTURE OF THE LECTIN MPA COMPLEXED WITH T-ANTIGEN DISACCHARIDE==
Maclura pomifera agglutinin is a tetrameric plant seed lectin with high affinity for the tumor-associated T-antigen disaccharide, Galbeta1,3GalNAcalpha, and hence for many O-linked glycopeptide structures. Unlike members of most lectin families, it lacks both metal ions and Cys residues. The structure of its complex with Galbeta1,3GalNAc was determined to 2.2 by first using multiwavelength anomalous diffraction with a lead derivative of the native protein, and then using molecular replacement with the unrefined structure as a model to solve the structure of the complex. The subunits share the beta-prism architecture and three-fold pseudo-symmetry of the related lectin jacalin, with the 21-residue beta-chains in the center of the tetramer. Interactions with the GalNAc predominate in the binding of the disaccharide. It forms a network of H-bonds with only one side chain, from an Asp residue, the amino group of the N-terminal Gly of the alpha-chain, and peptide backbone atoms of two aromatic residues. The Gal moiety does not H-bond directly with residues in the same monomer, i.e. there is no true subsite for it, but there are interactions through two water molecules. In the crystal, it interacts with residues in the binding site of an adjacent tetramer. The minimum energy conformation expected for the disaccharide is retained, despite its mediating the tetramer-tetramer interactions in the crystal packing. The resulting lattice is comparable to those seen for complexes of other lectins with branched glycopeptides.
<StructureSection load='1jot' size='340' side='right'caption='[[1jot]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jot]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Maclura_pomifera Maclura pomifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JOT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900084:Thomsen-Friedenreich+antigen'>PRD_900084</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jot OCA], [https://pdbe.org/1jot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jot RCSB], [https://www.ebi.ac.uk/pdbsum/1jot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jot ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECA_MACPO LECA_MACPO] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/1jot_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jot ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JOT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Maclura_pomifera Maclura pomifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOT OCA].
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of the complex of Maclura pomifera agglutinin and the T-antigen disaccharide, Galbeta1,3GalNAc., Lee X, Thompson A, Zhang Z, Ton-that H, Biesterfeldt J, Ogata C, Xu L, Johnston RA, Young NM, J Biol Chem. 1998 Mar 13;273(11):6312-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9497359 9497359]
[[Category: Large Structures]]
[[Category: Maclura pomifera]]
[[Category: Maclura pomifera]]
[[Category: Protein complex]]
[[Category: Biesterfeldt J]]
[[Category: Biesterfeldt, J.]]
[[Category: Hoa T-T]]
[[Category: Hoa, T T.]]
[[Category: Johnston RAZ]]
[[Category: Johnston, R A.Z.]]
[[Category: Lee X]]
[[Category: Lee, X.]]
[[Category: Ogata C]]
[[Category: Ogata, C.]]
[[Category: Thompson A]]
[[Category: Thompson, A.]]
[[Category: Xu L]]
[[Category: Xu, L.]]
[[Category: Young NM]]
[[Category: Young, N M.]]
[[Category: Zhang Z]]
[[Category: Zhang, Z.]]
[[Category: beta prism]]
[[Category: lectin]]
[[Category: maclura pomifera]]
[[Category: multi-wavelength anomalous diffraction (mad)]]
[[Category: t-antigen]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:00 2008''

Latest revision as of 10:41, 7 February 2024

STRUCTURE OF THE LECTIN MPA COMPLEXED WITH T-ANTIGEN DISACCHARIDESTRUCTURE OF THE LECTIN MPA COMPLEXED WITH T-ANTIGEN DISACCHARIDE

Structural highlights

1jot is a 2 chain structure with sequence from Maclura pomifera. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LECA_MACPO D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1jot, resolution 2.20Å

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