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[[Image:1jnu.gif|left|200px]]<br /><applet load="1jnu" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1jnu, resolution 2.60&Aring;" />
'''Photoexcited structure of the plant photoreceptor domain, phy3 LOV2'''<br />


==Overview==
==Photoexcited structure of the plant photoreceptor domain, phy3 LOV2==
The phototropins are flavoprotein kinases that control phototropic bending, light-induced chloroplast movement, and stomatal opening in plants. Two flavin mononucleotide binding light, oxygen, or voltage (LOV) domains are the sites for initial photochemistry in these blue light photoreceptors. We have determined the steady state, photoexcited crystal structure of a flavin-bound LOV domain. The structure reveals a unique photochemical switch in the flavin binding pocket in which the absorption of light drives the formation of a reversible covalent bond between a highly conserved Cys residue and the flavin cofactor. This provides a molecular picture of a cysteinyl-flavin covalent adduct, the presumed signaling species that leads to phototropin kinase activation and subsequent signal transduction. We identify closely related LOV domains in two eubacterial proteins that suggests the light-induced conformational change evident in this structure is an ancient biomolecular response to light, arising before the appearance of plants.
<StructureSection load='1jnu' size='340' side='right'caption='[[1jnu]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1jnu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Adiantum_capillus-veneris Adiantum capillus-veneris]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JNU FirstGlance]. <br>
1JNU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNU OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jnu OCA], [https://pdbe.org/1jnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jnu RCSB], [https://www.ebi.ac.uk/pdbsum/1jnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jnu ProSAT]</span></td></tr>
Photoexcited structure of a plant photoreceptor domain reveals a light-driven molecular switch., Crosson S, Moffat K, Plant Cell. 2002 May;14(5):1067-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12034897 12034897]
</table>
[[Category: Eukaryota]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q9ZWQ6_ADICA Q9ZWQ6_ADICA]  
[[Category: Crosson, S.]]
== Evolutionary Conservation ==
[[Category: Moffat, K.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: FMN]]
Check<jmol>
[[Category: cysteinyl-flavin adduct]]
  <jmolCheckbox>
[[Category: light-driven bond]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/1jnu_consurf.spt"</scriptWhenChecked>
[[Category: lov]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: pas]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: photochemistry]]
  </jmolCheckbox>
[[Category: photoexcited]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jnu ConSurf].
[[Category: phototropin]]
<div style="clear:both"></div>
[[Category: phy3]]
__TOC__
[[Category: plant photoreceptor]]
</StructureSection>
 
[[Category: Adiantum capillus-veneris]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:50 2008''
[[Category: Large Structures]]
[[Category: Phototropin]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Crosson S]]
[[Category: Moffat K]]

Latest revision as of 09:04, 3 April 2024

Photoexcited structure of the plant photoreceptor domain, phy3 LOV2Photoexcited structure of the plant photoreceptor domain, phy3 LOV2

Structural highlights

1jnu is a 4 chain structure with sequence from Adiantum capillus-veneris. The March 2015 RCSB PDB Molecule of the Month feature on Phototropin by David Goodsell is 10.2210/rcsb_pdb/mom_2015_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9ZWQ6_ADICA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1jnu, resolution 2.60Å

Drag the structure with the mouse to rotate

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OCA
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