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[[Image:1j2p.gif|left|200px]]<br /><applet load="1j2p" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1j2p, resolution 2.60&Aring;" />
'''alpha-ring from the proteasome from archaeoglobus fulgidus'''<br />


==Overview==
==alpha-ring from the proteasome from archaeoglobus fulgidus==
The 20S proteasome (core particle, CP) is a multifunctional protease complex and composed of four heptameric subunit rings arranged in a hollow, barrel-shaped structure. Here, we report the crystal structure of the CP from Archaeoglobus fulgidus at 2.25A resolution. The analysis of the structure of early and late assembly intermediates of this CP gives new insights in the maturation of archaebacterial CPs and indicates similarities to assembly intermediates observed in eukaryotes. We also show a striking difference in mechanism and regulation of substrate access between eukaryotic and archaebacterial 20S proteasomes. While eukaryotic CPs are auto-inhibited by the N-terminal tails of the outer alpha-ring by imposing topological closure with a characteristic sequence motif (YDR-motif) and show regulatory gating this segment is disordered in the CP and differently structured in the alpha(7)-sub-complex of A.fulgidus leaving a pore leading into the particle with a diameter of 13A. Mutagenesis and functional studies indicate the absence of regulatory gating in the archaeal 20S proteasome.
<StructureSection load='1j2p' size='340' side='right'caption='[[1j2p]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1j2p]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J2P FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j2p OCA], [https://pdbe.org/1j2p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j2p RCSB], [https://www.ebi.ac.uk/pdbsum/1j2p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j2p ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PSA_ARCFU PSA_ARCFU] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation (By similarity).[HAMAP-Rule:MF_00289_A]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j2/1j2p_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j2p ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1J2P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2P OCA].
*[[Proteasome 3D structures|Proteasome 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Investigations on the maturation and regulation of archaebacterial proteasomes., Groll M, Brandstetter H, Bartunik H, Bourenkow G, Huber R, J Mol Biol. 2003 Mar 14;327(1):75-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12614609 12614609]
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Proteasome endopeptidase complex]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Bartunik H]]
[[Category: Bartunik, H.]]
[[Category: Bourenkow G]]
[[Category: Bourenkow, G.]]
[[Category: Brandstetter H]]
[[Category: Brandstetter, H.]]
[[Category: Groll M]]
[[Category: Groll, M.]]
[[Category: Huber R]]
[[Category: Huber, R.]]
[[Category: alpha-ring]]
[[Category: proteasome]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:18:17 2008''

Latest revision as of 09:03, 3 April 2024

alpha-ring from the proteasome from archaeoglobus fulgidusalpha-ring from the proteasome from archaeoglobus fulgidus

Structural highlights

1j2p is a 7 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PSA_ARCFU Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation (By similarity).[HAMAP-Rule:MF_00289_A]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1j2p, resolution 2.60Å

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