|
|
| (13 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:1ih1.gif|left|200px]]<br /><applet load="1ih1" size="350" color="white" frame="true" align="right" spinBox="true"
| |
| caption="1ih1, resolution 2.0Å" />
| |
| '''Crystal Structure of the B-DNA Hexamer GGCGCC with Cobalt Hexamine Resolved to 2.0 Angstroms'''<br />
| |
|
| |
|
| ==Overview== | | ==Crystal Structure of the B-DNA Hexamer GGCGCC with Cobalt Hexamine Resolved to 2.0 Angstroms== |
| The transition between B- and A-DNA was first observed nearly 50 years ago. We have now mapped this transformation through a set of single-crystal structures of the sequence d(GGCGCC)2, with various intermediates being trapped by methylating or brominating the cytosine bases. The resulting pathway progresses through 13 conformational steps, with a composite structure that pairs A-nucleotides with complementary B-nucleotides serving as a distinct transition intermediate. The details of each step in the conversion of B- to A-DNA are thus revealed at the atomic level, placing intermediates for this and other sequences in the context of a common pathway.
| | <StructureSection load='1ih1' size='340' side='right'caption='[[1ih1]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1ih1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IH1 FirstGlance]. <br> |
| 1IH1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IH1 OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ih1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ih1 OCA], [https://pdbe.org/1ih1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ih1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ih1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ih1 ProSAT]</span></td></tr> |
| A crystallographic map of the transition from B-DNA to A-DNA., Vargason JM, Henderson K, Ho PS, Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7265-70. Epub 2001 Jun 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11390969 11390969]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Henderson, K.]]
| | </StructureSection> |
| [[Category: Ho, P S.]]
| | [[Category: Large Structures]] |
| [[Category: Vargason, J M.]]
| | [[Category: Henderson K]] |
| [[Category: MG]]
| | [[Category: Ho PS]] |
| [[Category: NCO]] | | [[Category: Vargason JM]] |
| [[Category: b to a dna transition]] | |
| [[Category: dna transition]] | |
| [[Category: structural transition]] | |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:45 2008''
| |