3lb4: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:3lb4.png|left|200px]]
-{{STRUCTURE_3lb4|  PDB=3lb4  |  SCENE=  }}
+==Two-site competitive inhibition in dehaloperoxidase-hemoglobin==
+<StructureSection load='3lb4' size='340' side='right'caption='[[3lb4]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3lb4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Amphitrite_ornata Amphitrite ornata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LB4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FPN:4-FLUOROPHENOL'>FPN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lb4 OCA], [https://pdbe.org/3lb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lb4 RCSB], [https://www.ebi.ac.uk/pdbsum/3lb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lb4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9NAV8_9ANNE Q9NAV8_9ANNE]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Dehaloperoxidase (DHP) from the annelid Amphitrite ornata is a catalytically active hemoglobin-peroxidase that possesses a unique internal binding cavity in the distal pocket above the heme. The previously published crystal structure of DHP shows 4-iodophenol bound internally. This led to the proposal that the internal binding site is the active site for phenol oxidation. However, the native substrate for DHP is 2,4,6-tribromophenol, and all attempts to bind 2,4,6-tribromophenol in the internal site under physiological conditions have failed. Herein, we show that the binding of 4-halophenols in the internal pocket inhibits enzymatic function. Furthermore, we demonstrate that DHP has a unique two-site competitive binding mechanism in which the internal and external binding sites communicate through two conformations of the distal histidine of the enzyme, resulting in nonclassical competitive inhibition. The same distal histidine conformations involved in DHP function regulate oxygen binding and release during transport and storage by hemoglobins and myoglobins. This work provides further support for the hypothesis that DHP possesses an external binding site for substrate oxidation, as is typical for the peroxidase family of enzymes.
-===Two-site competitive inhibition in dehaloperoxidase-hemoglobin===
+Internal binding of halogenated phenols in dehaloperoxidase-hemoglobin inhibits peroxidase function.,Thompson MK, Davis MF, de Serrano V, Nicoletti FP, Howes BD, Smulevich G, Franzen S Biophys J. 2010 Sep 8;99(5):1586-95. PMID:20816071<ref>PMID:20816071</ref>
-{{ABSTRACT_PUBMED_20816071}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3lb4" style="background-color:#fffaf0;"></div>
-[[3lb4]] is a 2 chain structure of [[Dehaloperoxidase]] and [[Hemoglobin]] with sequence from [http://en.wikipedia.org/wiki/Amphitrite_ornata Amphitrite ornata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LB4 OCA].
 ==See Also==
-*[[Dehaloperoxidase|Dehaloperoxidase]]
+*[[Dehaloperoxidase 3D structures|Dehaloperoxidase 3D structures]]
-*[[Hemoglobin|Hemoglobin]]
+*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:020816071</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Amphitrite ornata]]
-[[Category: Davis, M F.]]
+[[Category: Large Structures]]
-[[Category: Franzen, S.]]
+[[Category: Davis MF]]
-[[Category: Howes, B D.]]
+[[Category: Franzen S]]
-[[Category: Nicoletti, F P.]]
+[[Category: Howes BD]]
-[[Category: Serrano, V S.de.]]
+[[Category: Nicoletti FP]]
-[[Category: Smulevich, G.]]
+[[Category: Smulevich G]]
-[[Category: Thompson, M K.]]
+[[Category: Thompson MK]]
-[[Category: Globin]]
+[[Category: De Serrano VS]]
-[[Category: Heme]]
-[[Category: Oxidoreductase]]
-[[Category: Oxygen transport]]
-[[Category: Peroxidase]]
-[[Category: Transport]]