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[[Image:1na6.png|left|200px]]


{{STRUCTURE_1na6|  PDB=1na6  |  SCENE=  }}
==Crystal structure of restriction endonuclease EcoRII mutant R88A==
 
<StructureSection load='1na6' size='340' side='right'caption='[[1na6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
===Crystal structure of restriction endonuclease EcoRII mutant R88A===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[1na6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NA6 FirstGlance]. <br>
{{ABSTRACT_PUBMED_14659759}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1na6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1na6 OCA], [https://pdbe.org/1na6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1na6 RCSB], [https://www.ebi.ac.uk/pdbsum/1na6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1na6 ProSAT]</span></td></tr>
==About this Structure==
</table>
[[1na6]] is a 2 chain structure of [[Endonuclease]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA6 OCA].  
== Function ==
[https://www.uniprot.org/uniprot/T2E2_ECOLX T2E2_ECOLX] Recognizes the double-stranded sequence CCWGG and cleaves before C-1.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1na6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1na6 ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
<ref group="xtra">PMID:014659759</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Large Structures]]
[[Category: Chen, L.]]
[[Category: Chen L]]
[[Category: Kruger, D H.]]
[[Category: Kruger DH]]
[[Category: Meehan, E J.]]
[[Category: Meehan EJ]]
[[Category: Mucke, M.]]
[[Category: Mucke M]]
[[Category: Reuter, M.]]
[[Category: Reuter M]]
[[Category: Wang, Y.]]
[[Category: Wang Y]]
[[Category: Zhou, X E.]]
[[Category: Zhou XE]]
[[Category: Hydrolase]]
[[Category: Mutation]]
[[Category: Replication]]
[[Category: Site-specific restriction]]

Latest revision as of 10:54, 14 February 2024

Crystal structure of restriction endonuclease EcoRII mutant R88ACrystal structure of restriction endonuclease EcoRII mutant R88A

Structural highlights

1na6 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

T2E2_ECOLX Recognizes the double-stranded sequence CCWGG and cleaves before C-1.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1na6, resolution 2.10Å

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