1g1m: Difference between revisions

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-[[Image:1g1m.jpg|left|200px]]<br /><applet load="1g1m" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1g1m, resolution 2.25&Aring;" />
-'''ALL-FERROUS NITROGENASE IRON PROTEIN FROM AZOTOBACTER VINELANDII'''<br />
-==Overview==
+==ALL-FERROUS NITROGENASE IRON PROTEIN FROM AZOTOBACTER VINELANDII==
-The structure of the nitrogenase iron protein from Azotobacter vinelandii in the all-ferrous [4Fe-4S](0) form has been determined to 2.25 A resolution by using the multiwavelength anomalous diffraction (MAD) phasing technique. The structure demonstrates that major conformational changes are not necessary either in the iron protein or in the cluster to accommodate cluster reduction to the [4Fe-4S](0) oxidation state. A survey of [4Fe-4S] clusters coordinated by four cysteine ligands in proteins of known structure reveals that the [4Fe-4S] cluster of the iron protein has the largest accessible surface area, suggesting that solvent exposure may be relevant to the ability of the iron protein to exist in three oxidation states.
+<StructureSection load='1g1m' size='340' side='right'caption='[[1g1m]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1g1m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G1M FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g1m OCA], [https://pdbe.org/1g1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g1m RCSB], [https://www.ebi.ac.uk/pdbsum/1g1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g1m ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NIFH1_AZOVI NIFH1_AZOVI] The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein.[HAMAP-Rule:MF_00533]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g1/1g1m_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g1m ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-G1M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G1M OCA].
+*[[Nitrogenase 3D structures|Nitrogenase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of the all-ferrous [4Fe-4S]0 form of the nitrogenase iron protein from Azotobacter vinelandii., Strop P, Takahara PM, Chiu H, Angove HC, Burgess BK, Rees DC, Biochemistry. 2001 Jan 23;40(3):651-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11170381 11170381]
 [[Category: Azotobacter vinelandii]]
-[[Category: Nitrogenase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Angove HC]]
-[[Category: Angove, H C.]]
+[[Category: Burgess BK]]
-[[Category: Burgess, B K.]]
+[[Category: Chiu H-J]]
-[[Category: Chiu, H J.]]
+[[Category: Rees DC]]
-[[Category: Rees, D C.]]
+[[Category: Strop P]]
-[[Category: Strop, P.]]
+[[Category: Takahara PM]]
-[[Category: Takahara, P M.]]
-[[Category: SF4]]
-[[Category: all-ferrous]]
-[[Category: iron protein]]
-[[Category: nitrogenase]]
-[[Category: oxidoreductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:45:01 2008''