2kbb: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2kbb.png|left|200px]]
-{{STRUCTURE_2kbb|  PDB=2kbb  |  SCENE=  }}
+==Solution Structure of the R9 Domain of Talin==
+<StructureSection load='2kbb' size='340' side='right'caption='[[2kbb]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2kbb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KBB FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kbb OCA], [https://pdbe.org/2kbb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kbb RCSB], [https://www.ebi.ac.uk/pdbsum/2kbb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kbb ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kbb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kbb ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Talin is a large flexible rod-shaped protein that activates the integrin family of cell adhesion molecules and couples them to cytoskeletal actin. It exists in both globular and extended conformations, and an intramolecular interaction between the N-terminal F3 FERM subdomain and the C-terminal part of the talin rod contributes to an autoinhibited form of the molecule. Here, we report the solution structure of the primary F3 binding domain within the C-terminal region of the talin rod and use intermolecular nuclear Overhauser effects to determine the structure of the complex. The rod domain (residues 1655-1822) is an amphipathic five-helix bundle; Tyr-377 of F3 docks into a hydrophobic pocket at one end of the bundle, whereas a basic loop in F3 (residues 316-326) interacts with a cluster of acidic residues in the middle of helix 4. Mutation of Glu-1770 abolishes binding. The rod domain competes with beta3-integrin tails for binding to F3, and the structure of the complex suggests that the rod is also likely to sterically inhibit binding of the FERM domain to the membrane.
-===NMR structure of the talin rod domain, 1655-1822===
+The structure of an interdomain complex that regulates talin activity.,Goult BT, Bate N, Anthis NJ, Wegener KL, Gingras AR, Patel B, Barsukov IL, Campbell ID, Roberts GC, Critchley DR J Biol Chem. 2009 May 29;284(22):15097-106. Epub 2009 Mar 18. PMID:19297334<ref>PMID:19297334</ref>
-{{ABSTRACT_PUBMED_19297334}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2kbb" style="background-color:#fffaf0;"></div>
-[[2kbb]] is a 1 chain structure of [[Talin]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBB OCA].
 ==See Also==
-*[[Talin|Talin]]
+*[[Talin 3D structures|Talin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019297334</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Barsukov, I L.]]
+[[Category: Barsukov IL]]
-[[Category: Bate, N.]]
+[[Category: Bate N]]
-[[Category: Critchley, D R.]]
+[[Category: Critchley DR]]
-[[Category: Gingras, A R.]]
+[[Category: Gingras AR]]
-[[Category: Goult, B T.]]
+[[Category: Goult BT]]
-[[Category: Autoinhibition]]
-[[Category: Bundle]]
-[[Category: Cell membrane]]
-[[Category: Cell projection]]
-[[Category: Cytoskeleton]]
-[[Category: F3]]
-[[Category: Integrin]]
-[[Category: Membrane]]
-[[Category: Phosphoprotein]]
-[[Category: Rod]]
-[[Category: Structural protein]]
-[[Category: Talin]]