1fbu: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1fbu.jpg|left|200px]]<br /><applet load="1fbu" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1fbu, resolution 2.00&Aring;" />
'''HEAT SHOCK TRANSCRIPTION FACTOR DNA BINDING DOMAIN'''<br />


==Overview==
==HEAT SHOCK TRANSCRIPTION FACTOR DNA BINDING DOMAIN==
The DNA-binding domain of the yeast heat shock transcription factor (HSF) contains a strictly conserved proline that is at the center of a kink. To define the role of this conserved proline-centered kink, we replaced the proline with a number of other residues. These substitutions did not diminish the ability of the full-length protein to support growth of yeast or to activate transcription, suggesting that the proline at the center of the kink is not conserved for function. The stability of the isolated mutant DNA-binding domains was unaltered from the wild-type, so the proline is not conserved to maintain the stability of the protein. The crystal structures of two of the mutant DNA-binding domains revealed that the helices in the mutant proteins were still kinked after substitution of the proline, suggesting that the proline does not cause the alpha-helical kink. So why are prolines conserved in this and the majority of other kinked alpha-helices if not for structure, function, or stability? The mutant DNA-binding domains are less soluble than wild-type when overexpressed. In addition, the folding kinetics, as measured by stopped-flow fluorescence, is faster for the mutant proteins. These two results support the premise that the presence of the proline is critical for the folding pathway of HSF's DNA-binding domain. The finding may also be more general and explain why kinked helices maintain their prolines.
<StructureSection load='1fbu' size='340' side='right'caption='[[1fbu]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1fbu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FBU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fbu OCA], [https://pdbe.org/1fbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fbu RCSB], [https://www.ebi.ac.uk/pdbsum/1fbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fbu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HSF_KLULA HSF_KLULA] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/1fbu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fbu ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1FBU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FBU OCA].
*[[Heat shock factor|Heat shock factor]]
 
__TOC__
==Reference==
</StructureSection>
Proline in alpha-helical kink is required for folding kinetics but not for kinked structure, function, or stability of heat shock transcription factor., Hardy JA, Nelson HC, Protein Sci. 2000 Nov;9(11):2128-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11305238 11305238]
[[Category: Kluyveromyces lactis]]
[[Category: Kluyveromyces lactis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Hardy, J A.]]
[[Category: Hardy JA]]
[[Category: Nelson, H C.M.]]
[[Category: Nelson HCM]]
[[Category: helical bulge]]
[[Category: helical kink]]
[[Category: helix-turn-helix]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:03 2008''

Latest revision as of 10:13, 7 February 2024

HEAT SHOCK TRANSCRIPTION FACTOR DNA BINDING DOMAINHEAT SHOCK TRANSCRIPTION FACTOR DNA BINDING DOMAIN

Structural highlights

1fbu is a 2 chain structure with sequence from Kluyveromyces lactis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HSF_KLULA DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. Also required for growth at normal temperatures.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1fbu, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1fbu&oldid=4043347"