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[[Image:1ewa.gif|left|200px]]<br /><applet load="1ewa" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1ewa, resolution 2.5&Aring;" />
'''Dehaloperoxidase and 4-iodophenol'''<br />


==Overview==
==Dehaloperoxidase and 4-iodophenol==
The full-length, protein coding sequence for dehaloperoxidase was obtained using a reverse genetic approach and a cDNA library from marine worm Amphitrite ornata. The crystal structure of the dehaloperoxidase (DHP) was determined by the multiple isomorphous replacement method and was refined at 1.8-A resolution. The enzyme fold is that of the globin family and, together with the amino acid sequence information, indicates that the enzyme evolved from an ancient oxygen carrier. The peroxidase activity of DHP arose mainly through changes in the positions of the proximal and distal histidines relative to those seen in globins. The structure of a complex of DHP with 4-iodophenol is also reported, and it shows that in contrast to larger heme peroxidases DHP binds organic substrates in the distal cavity. The binding is facilitated by the histidine swinging in and out of the cavity. The modeled position of the oxygen atom bound to the heme suggests that the enzymatic reaction proceeds via direct attack of the oxygen atom on the carbon atom bound to the halogen atom.
<StructureSection load='1ewa' size='340' side='right'caption='[[1ewa]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ewa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Amphitrite_ornata Amphitrite ornata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IOL:4-IODOPHENOL'>IOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ewa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ewa OCA], [https://pdbe.org/1ewa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ewa RCSB], [https://www.ebi.ac.uk/pdbsum/1ewa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ewa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9NAV8_9ANNE Q9NAV8_9ANNE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ewa_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ewa ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EWA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Amphitrite_ornata Amphitrite ornata] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=IOL:'>IOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWA OCA].
*[[Dehaloperoxidase 3D structures|Dehaloperoxidase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure and amino acid sequence of dehaloperoxidase from Amphitrite ornata indicate common ancestry with globins., LaCount MW, Zhang E, Chen YP, Han K, Whitton MM, Lincoln DE, Woodin SA, Lebioda L, J Biol Chem. 2000 Jun 23;275(25):18712-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10751397 10751397]
[[Category: Amphitrite ornata]]
[[Category: Amphitrite ornata]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chen, Y P.]]
[[Category: Chen YP]]
[[Category: Han, K.]]
[[Category: Han K]]
[[Category: LaCount, M W.]]
[[Category: LaCount MW]]
[[Category: Lebioda, L.]]
[[Category: Lebioda L]]
[[Category: Lincoln, D E.]]
[[Category: Lincoln DE]]
[[Category: Whitton, M M.]]
[[Category: Whitton MM]]
[[Category: Woodin, S A.]]
[[Category: Woodin SA]]
[[Category: Zhang, E.]]
[[Category: Zhang E]]
[[Category: HEM]]
[[Category: IOL]]
[[Category: SO4]]
[[Category: globin]]
[[Category: peroxidase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:32:19 2008''

Latest revision as of 10:07, 7 February 2024

Dehaloperoxidase and 4-iodophenolDehaloperoxidase and 4-iodophenol

Structural highlights

1ewa is a 2 chain structure with sequence from Amphitrite ornata. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9NAV8_9ANNE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1ewa, resolution 2.50Å

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