1eod: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1eod.gif|left|200px]]<br /><applet load="1eod" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1eod, resolution 2.45&Aring;" />
-'''CRYSTAL STRUCTURE OF THE N136D MUTANT OF A SHAKER T1 DOMAIN'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE N136D MUTANT OF A SHAKER T1 DOMAIN==
-The T1 domain, a highly conserved cytoplasmic portion at the N-terminus of the voltage-dependent K+ channel (Kv) alpha-subunit, is responsible for driving and regulating the tetramerization of the alpha-subunits. Here we report the identification of a set of mutations in the T1 domain that alter the gating properties of the Kv channel. Two mutants produce a leftward shift in the activation curve and slow the channel closing rate while a third mutation produces a rightward shift in the activation curve and speeds the channel closing rate. We have determined the crystal structures of T1 domains containing these mutations. Both of the leftward shifting mutants produce similar conformational changes in the putative membrane facing surface of the T1 domain. These results suggest that the structure of the T1 domain in this region is tightly coupled to the channel's gating states.
+<StructureSection load='1eod' size='340' side='right'caption='[[1eod]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1eod]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOD FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eod OCA], [https://pdbe.org/1eod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eod RCSB], [https://www.ebi.ac.uk/pdbsum/1eod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eod ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q16968_APLCA Q16968_APLCA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eod_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eod ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-EOD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOD OCA].
+*[[Potassium channel 3D structures|Potassium channel 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Voltage dependent activation of potassium channels is coupled to T1 domain structure., Cushman SJ, Nanao MH, Jahng AW, DeRubeis D, Choe S, Pfaffinger PJ, Nat Struct Biol. 2000 May;7(5):403-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10802739 10802739]
 [[Category: Aplysia californica]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Choe, S.]]
+[[Category: Choe S]]
-[[Category: Cushman, S J.]]
+[[Category: Cushman SJ]]
-[[Category: DeRubeis, D.]]
+[[Category: DeRubeis D]]
-[[Category: Jahng, A W.]]
+[[Category: Jahng AW]]
-[[Category: Nanao, M H.]]
+[[Category: Nanao MH]]
-[[Category: Pfaffinger, P J.]]
+[[Category: Pfaffinger PJ]]
-[[Category: aplysia kv1 1]]
-[[Category: potassium channels]]
-[[Category: proton transport]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:55 2008''