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[[Image:1elq.gif|left|200px]]<br /><applet load="1elq" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1elq, resolution 1.80&Aring;" />
'''CRYSTAL STRUCTURE OF THE CYSTINE C-S LYASE C-DES'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE CYSTINE C-S LYASE C-DES==
FeS clusters are versatile cofactors of a variety of proteins, but the mechanisms of their biosynthesis are still unknown. The cystine C-S lyase from Synechocystis has been identified as a participant in ferredoxin FeS cluster formation. Herein, we report on the crystal structure of the lyase and of a complex with the reaction products of cystine cleavage at 1.8- and 1.55-A resolution, respectively. The sulfur-containing product was unequivocally identified as cysteine persulfide. The reactive persulfide group is fixed by a hydrogen bond to His-114 in the center of a hydrophobic pocket and is thereby shielded from the solvent. Binding and stabilization of the cysteine persulfide represent an alternative to the generation of a protein-bound persulfide by NifS-like proteins and point to the general importance of persulfidic compounds for FeS cluster assembly.
<StructureSection load='1elq' size='340' side='right'caption='[[1elq]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1elq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6714 Synechocystis sp. PCC 6714]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELQ FirstGlance]. <br>
1ELQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELQ OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elq OCA], [https://pdbe.org/1elq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elq RCSB], [https://www.ebi.ac.uk/pdbsum/1elq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elq ProSAT]</span></td></tr>
Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis., Clausen T, Kaiser JT, Steegborn C, Huber R, Kessler D, Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3856-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10760256 10760256]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Synechocystis sp.]]
[https://www.uniprot.org/uniprot/Q9ZHG9_SYNY4 Q9ZHG9_SYNY4]  
[[Category: Clausen, T.]]
== Evolutionary Conservation ==
[[Category: Huber, R.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Kaiser, J T.]]
Check<jmol>
[[Category: Kessler, D.]]
  <jmolCheckbox>
[[Category: Steegborn, C.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elq_consurf.spt"</scriptWhenChecked>
[[Category: K]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: PLP]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: fes cluster biosynthesis]]
  </jmolCheckbox>
[[Category: nifs]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1elq ConSurf].
[[Category: pyridoxal 5'-phosphate]]
<div style="clear:both"></div>
[[Category: thiocysteine]]
__TOC__
 
</StructureSection>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:09 2008''
[[Category: Large Structures]]
[[Category: Synechocystis sp. PCC 6714]]
[[Category: Clausen T]]
[[Category: Huber R]]
[[Category: Kaiser JT]]
[[Category: Kessler D]]
[[Category: Steegborn C]]

Latest revision as of 13:04, 20 March 2024

CRYSTAL STRUCTURE OF THE CYSTINE C-S LYASE C-DESCRYSTAL STRUCTURE OF THE CYSTINE C-S LYASE C-DES

Structural highlights

1elq is a 2 chain structure with sequence from Synechocystis sp. PCC 6714. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9ZHG9_SYNY4

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1elq, resolution 1.80Å

Drag the structure with the mouse to rotate

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OCA
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