1eik: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1eik.gif|left|200px]]<br /><applet load="1eik" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1eik" />
-'''SOLUTION STRUCTURE OF RNA POLYMERASE SUBUNIT RPB5 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM'''<br />
-==Overview==
+==Solution Structure of RNA Polymerase Subunit RPB5 from Methanobacterium Thermoautotrophicum==
+<StructureSection load='1eik' size='340' side='right'caption='[[1eik]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1eik]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIK FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eik OCA], [https://pdbe.org/1eik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eik RCSB], [https://www.ebi.ac.uk/pdbsum/1eik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eik ProSAT], [https://www.topsan.org/Proteins/NESGC/1eik TOPSAN]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPO5_METTH RPO5_METTH] DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_00025]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eik_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eik ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
 RPB5 is an essential subunit of eukaryotic and archaeal RNA polymerases. It is a proposed target for transcription activator proteins in eukaryotes, but the mechanism of interaction is not known. We have determined the solution structure of the RPB5 subunit from the thermophilic archeon, Methanobacterium thermoautotrophicum. MtRBP5 contains a four-stranded beta-sheet platform supporting two alpha-helices, one on each side of the beta-sheet, resulting in an overall mushroom shape that does not appear to have any structural homologues in the structural database. The position and conservation of charged surface residues suggests possible modes of interaction with other proteins, as well as a rationale for the thermal stability of this protein.
-==About this Structure==
+Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum.,Yee A, Booth V, Dharamsi A, Engel A, Edwards AM, Arrowsmith CH Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6311-5. PMID:10841538<ref>PMID:10841538</ref>
-EIK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIK OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum., Yee A, Booth V, Dharamsi A, Engel A, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6311-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10841538 10841538]
+</div>
-[[Category: DNA-directed RNA polymerase]]
+<div class="pdbe-citations 1eik" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Methanothermobacter thermautotrophicus]]
-[[Category: Single protein]]
+[[Category: Arrowsmith CH]]
-[[Category: Arrowsmith, C H.]]
+[[Category: Booth V]]
-[[Category: Booth, V.]]
+[[Category: Dharamsi A]]
-[[Category: Dharamsi, A.]]
+[[Category: Edwards AM]]
-[[Category: Edwards, A M.]]
+[[Category: Engel A]]
-[[Category: Engel, A.]]
+[[Category: Yee A]]
-[[Category: NESG, Northeast Structural Genomics Consortium.]]
-[[Category: Yee, A.]]
-[[Category: nesg]]
-[[Category: northeast structural genomics consortium]]
-[[Category: ocsp]]
-[[Category: protein structure initiative]]
-[[Category: psi]]
-[[Category: rna polymerase subunit]]
-[[Category: rpb5]]
-[[Category: rpbh]]
-[[Category: structural genomics]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:09 2008''