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| [[Image:1duq.gif|left|200px]]<br /><applet load="1duq" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1duq, resolution 2.10Å" />
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| '''CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1'''<br />
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| ==Overview== | | ==CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1== |
| The crystal and molecular structure of an RNA duplex corresponding to the high affinity Rev protein binding element (RBE) has been determined at 2.1-A resolution. Four unique duplexes are present in the crystal, comprising two structural variants. In each duplex, the RNA double helix consists of an annealed 12-mer and 14-mer that form an asymmetric internal loop consisting of G-G and G-A noncanonical base pairs and a flipped-out uridine. The 12-mer strand has an A-form conformation, whereas the 14-mer strand is distorted to accommodate the bulges and noncanonical base pairing. In contrast to the NMR model of the unbound RBE, an asymmetric G-G pair with N2-N7 and N1-O6 hydrogen bonding, is formed in each helix. The G-A base pairing agrees with the NMR structure in one structural variant, but forms a novel water-mediated pair in the other. A backbone flip and reorientation of the G-G base pair is required to assume the RBE conformation present in the NMR model of the complex between the RBE and the Rev peptide.
| | <StructureSection load='1duq' size='340' side='right'caption='[[1duq]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1duq]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DUQ FirstGlance]. <br> |
| 1DUQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUQ OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1duq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1duq OCA], [https://pdbe.org/1duq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1duq RCSB], [https://www.ebi.ac.uk/pdbsum/1duq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1duq ProSAT]</span></td></tr> |
| The crystal structure of the Rev binding element of HIV-1 reveals novel base pairing and conformational variability., Hung LW, Holbrook EL, Holbrook SR, Proc Natl Acad Sci U S A. 2000 May 9;97(10):5107-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10792052 10792052]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Holbrook, E L.]] | | </StructureSection> |
| [[Category: Holbrook, S R.]] | | [[Category: Large Structures]] |
| [[Category: Hung, L W.]] | | [[Category: Holbrook EL]] |
| [[Category: NA]]
| | [[Category: Holbrook SR]] |
| [[Category: hiv-1]]
| | [[Category: Hung L-W]] |
| [[Category: rev binding domain]]
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| [[Category: rre]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:43 2008''
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