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| [[Image:1cx0.gif|left|200px]]<br /><applet load="1cx0" size="350" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[4pr6]] This PDB entry is obsolete and replaced by 4pr6 |
| caption="1cx0, resolution 2.30Å" />
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| '''HEPATITIS DELTA VIRUS RIBOZYME'''<br />
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| ==Overview==
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| The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.
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| ==About this Structure==
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| 1CX0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX0 OCA].
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| ==Reference==
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| Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9783582 9783582]
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| [[Category: Hepatitis delta virus]]
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| [[Category: Single protein]]
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| [[Category: Amare, A R.Ferre-D.]]
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| [[Category: Doudna, J A.]]
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| [[Category: Zhou, K.]]
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| [[Category: MG]]
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| [[Category: nested double pseudoknot rna structure]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:36 2008''
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