2vel: Difference between revisions

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-[[Image:2vel.png|left|200px]]
-{{STRUCTURE_2vel|  PDB=2vel  |  SCENE=  }}
+==Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties==
+<StructureSection load='2vel' size='340' side='right'caption='[[2vel]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-===STRUCTURE-BASED ENZYME ENGINEERING EFFORTS WITH AN INACTIVE MONOMERIC TIM VARIANT: THE IMPORTANCE OF A SINGLE POINT MUTATION FOR GENERATING AN ACTIVE SITE WITH SUITABLE BINDING PROPERTIES===
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2vel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VEL FirstGlance]. <br>
-{{ABSTRACT_PUBMED_18239072}}
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene></td></tr>
-==About this Structure==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vel OCA], [https://pdbe.org/2vel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vel RCSB], [https://www.ebi.ac.uk/pdbsum/2vel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vel ProSAT]</span></td></tr>
-[[2vel]] is a 2 chain structure of [[TIM test]] and [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VEL OCA].
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TPIS_TRYBB TPIS_TRYBB]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ve/2vel_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vel ConSurf].
+<div style="clear:both"></div>
 ==See Also==
-*[[TIM test|TIM test]]
+*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
-*[[Triose Phosphate Isomerase|Triose Phosphate Isomerase]]
+__TOC__
+</StructureSection>
-==Reference==
+[[Category: Large Structures]]
-<ref group="xtra">PMID:018239072</ref><references group="xtra"/>
-[[Category: Triose-phosphate isomerase]]
 [[Category: Trypanosoma brucei brucei]]
-[[Category: Alahuhta, M.]]
+[[Category: Alahuhta M]]
-[[Category: Augustyns, K.]]
+[[Category: Augustyns K]]
-[[Category: Casteleijn, M G.]]
+[[Category: Casteleijn MG]]
-[[Category: El-Sayed, I.]]
+[[Category: El-Sayed I]]
-[[Category: Kemmer, C.]]
+[[Category: Kemmer C]]
-[[Category: Neubauer, P.]]
+[[Category: Neubauer P]]
-[[Category: Salin, M.]]
+[[Category: Salin M]]
-[[Category: Wierenga, R K.]]
+[[Category: Wierenga RK]]
-[[Category: Binding pocket]]
-[[Category: Engineering]]
-[[Category: Enzyme]]
-[[Category: Fatty acid biosynthesis]]
-[[Category: Gluconeogenesis]]
-[[Category: Glycolysis]]
-[[Category: Glycosome]]
-[[Category: Isomerase]]
-[[Category: Lipid synthesis]]
-[[Category: Monomeric]]
-[[Category: Pentose shunt]]
-[[Category: Substrate specificity]]
-[[Category: Tim]]
-[[Category: Tim barrel]]
-[[Category: Triosephosphate isomerase]]