1au7: Difference between revisions

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-[[Image:1au7.gif|left|200px]]<br /><applet load="1au7" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1au7, resolution 2.300&Aring;" />
-'''PIT-1 MUTANT/DNA COMPLEX'''<br />
-==Overview==
+==PIT-1 MUTANT/DNA COMPLEX==
-Pit-1, a member of the POU domain family of transcription factors, characterized by a bipartite DNA-binding domain, serves critical developmental functions based on binding to diverse DNA elements in its target genes. Here we report a high resolution X-ray analysis of the Pit-1 POU domain bound to a DNA element as a homodimer. This analysis reveals that Pit-1 subdomains bind to perpendicular faces of the DNA, rather than opposite faces of the DNA as in Oct-1. This is accomplished by different spacing and orientation of the POU-specific domain. Contrary to previous predictions, the dimerization interface involves the carboxyl terminus of the DNA recognition helix of the homeodomain, which in an extended conformation interacts with specific residues at the amino terminus of helix alpha1 and in the loop between helices alpha3 and alpha4 of the POU-specific domain of the symmetry related monomer. These features suggest the molecular basis of disease-causing mutations in Pit-1 and provide potential basis for the flexible allostery between protein domains and DNA sites in the activation of target genes.
+<StructureSection load='1au7' size='340' side='right'caption='[[1au7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1au7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AU7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1au7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1au7 OCA], [https://pdbe.org/1au7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1au7 RCSB], [https://www.ebi.ac.uk/pdbsum/1au7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1au7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PIT1_RAT PIT1_RAT] Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1au7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1au7 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-AU7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AU7 OCA].
+*[[Art:Molecular Sculpture|Art:Molecular Sculpture]]
+__TOC__
-==Reference==
+</StructureSection>
-Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility., Jacobson EM, Li P, Leon-del-Rio A, Rosenfeld MG, Aggarwal AK, Genes Dev. 1997 Jan 15;11(2):198-212. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9009203 9009203]
+[[Category: Large Structures]]
 [[Category: Rattus norvegicus]]
-[[Category: Single protein]]
+[[Category: Aggarwal AK]]
-[[Category: Aggarwal, A K.]]
+[[Category: Jacobson EM]]
-[[Category: Jacobson, E M.]]
+[[Category: Leon-Del-Rio A]]
-[[Category: Leon-Del-Rio, A.]]
+[[Category: Li P]]
-[[Category: Li, P.]]
+[[Category: Rosenfeld MG]]
-[[Category: Rosenfeld, M G.]]
-[[Category: complex (dna-binding protein/dna)]]
-[[Category: cphd]]
-[[Category: pituitary]]
-[[Category: pou domain]]
-[[Category: transcription factor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:20 2008''