1a7d: Difference between revisions

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-[[Image:1a7d.gif|left|200px]]<br /><applet load="1a7d" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1a7d, resolution 1.8&Aring;" />
-'''CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA'''<br />
-==Overview==
+==CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA==
-Myohemerythrin (Mhr) is a nonheme iron oxygen carrier found in the retractor muscles of marine "peanut" worms. The X-ray crystal structures of two recombinant Themiste zostericola Mhrs are reported to a resolution of 1.8 A. Surprisingly, the met wild-type structure (R = 17.8%) was found to contain chloride bound to Fe2, while coordinated hydroxide was found in the met L103N structure (R = 18.3%). An internal water molecule was also found distal to the Fe-O-Fe center of the mutant protein, forming hydrogen bonds with the coordinated hydroxide and the OD1 atom of Asn-103. This finding is consistent with the kinetic and spectroscopic results reported for the L103N mutant Mhr [Raner, G. M., Martins, L. J., &amp; Ellis, W. R., Jr. (1997) Biochemistry 36, 7037-7043]. Possible roles for the side chain of residue 103 (Leu in wild-type Mhr) in gating ligand binding are also discussed.
+<StructureSection load='1a7d' size='340' side='right'caption='[[1a7d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1a7d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Themiste_hennahi Themiste hennahi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A7D FirstGlance]. <br>
-A7D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Themiste_zostericola Themiste zostericola] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CFO:'>CFO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7D OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CFO:CHLORO+DIIRON-OXO+MOIETY'>CFO</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a7d OCA], [https://pdbe.org/1a7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a7d RCSB], [https://www.ebi.ac.uk/pdbsum/1a7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a7d ProSAT]</span></td></tr>
-Structures of wild-type chloromet and L103N hydroxomet Themiste zostericola myohemerythrins at 1.8 A resolution., Martins LJ, Hill CP, Ellis WR Jr, Biochemistry. 1997 Jun 10;36(23):7044-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9188702 9188702]
+</table>
-[[Category: Single protein]]
+== Function ==
-[[Category: Themiste zostericola]]
+[https://www.uniprot.org/uniprot/HEMTM_THEHE HEMTM_THEHE] Myohemerythrin is an oxygen-binding protein found in the retractor muscles of certain worms. The oxygen-binding site contains two iron atoms.
-[[Category: Hill, C P.]]
+== Evolutionary Conservation ==
-[[Category: Junior, W R.Ellis.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Martins, L J.]]
+Check<jmol>
-[[Category: CFO]]
+  <jmolCheckbox>
-[[Category: CL]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/1a7d_consurf.spt"</scriptWhenChecked>
-[[Category: nonheme iron oxygen carrier]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: oxygen transport]]
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:42 2008''
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a7d ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Themiste hennahi]]
+[[Category: Ellis Junior WR]]
+[[Category: Hill CP]]
+[[Category: Martins LJ]]