2ilk: Difference between revisions

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-[[Image:2ilk.jpg|left|200px]]<br /><applet load="2ilk" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2ilk, resolution 1.6&Aring;" />
-'''CRYSTAL STRUCTURE OF HUMAN INTERLEUKIN-10 AT 1.6 ANGSTROMS RESOLUTION'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF HUMAN INTERLEUKIN-10 AT 1.6 ANGSTROMS RESOLUTION==
-The crystal structure of human interleukin-10 (IL-10) was refined at 1.6 A, resolution against X-ray diffraction data collected at 100 K with the use, of synchrotron radiation. Although similar to the IL-10 structure, determined previously at room temperature, this low-temperature IL-10, structure contains, in addition, four N-terminal residues, three sulfate, anions, and 175 extra water molecules. Whereas the main-chain conformation, is preserved, about 30% of the side chains, most of them on the protein, surface, assume different conformations. A computer model of a complex of, IL-10 with its two soluble receptors was generated based on the, topological similarity of IL-10 to interferon-gamma. The contact region, between the cytokine and each receptor shows excellent complementarity of, polar and hydrophobic interactions, suggesting that the model is generally, correct and should be useful in guiding mutagenesis experiments.
+<StructureSection load='2ilk' size='340' side='right'caption='[[2ilk]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ilk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ILK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ILK FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ilk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ilk OCA], [https://pdbe.org/2ilk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ilk RCSB], [https://www.ebi.ac.uk/pdbsum/2ilk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ilk ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[https://www.uniprot.org/uniprot/IL10_HUMAN IL10_HUMAN]] Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/il/2ilk_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ilk ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of human interleukin-10 (IL-10) was refined at 1.6 A resolution against X-ray diffraction data collected at 100 K with the use of synchrotron radiation. Although similar to the IL-10 structure determined previously at room temperature, this low-temperature IL-10 structure contains, in addition, four N-terminal residues, three sulfate anions, and 175 extra water molecules. Whereas the main-chain conformation is preserved, about 30% of the side chains, most of them on the protein surface, assume different conformations. A computer model of a complex of IL-10 with its two soluble receptors was generated based on the topological similarity of IL-10 to interferon-gamma. The contact region between the cytokine and each receptor shows excellent complementarity of polar and hydrophobic interactions, suggesting that the model is generally correct and should be useful in guiding mutagenesis experiments.
-==Disease==
+Crystal structure of human interleukin-10 at 1.6 A resolution and a model of a complex with its soluble receptor.,Zdanov A, Schalk-Hihi C, Wlodawer A Protein Sci. 1996 Oct;5(10):1955-62. PMID:8897595<ref>PMID:8897595</ref>
-Known diseases associated with this structure: Graft-versus-host disease, protection against OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=124092 124092]], HIV-1, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=124092 124092]], Rheumatoid arthritis, progression of OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=124092 124092]]
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-ILK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ILK OCA].
+</div>
+<div class="pdbe-citations 2ilk" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Crystal structure of human interleukin-10 at 1.6 A resolution and a model of a complex with its soluble receptor., Zdanov A, Schalk-Hihi C, Wlodawer A, Protein Sci. 1996 Oct;5(10):1955-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8897595 8897595]
+*[[Interleukin 3D structures|Interleukin 3D structures]]
-[[Category: Homo sapiens]]
+== References ==
-[[Category: Single protein]]
+<references/>
-[[Category: Schalk-Hihi, C.]]
+__TOC__
-[[Category: Wlodawer, A.]]
+</StructureSection>
-[[Category: Zdanov, A.]]
+[[Category: Human]]
-[[Category: SO4]]
+[[Category: Large Structures]]
-[[Category: cytokine]]
+[[Category: Schalk-Hihi, C]]
+[[Category: Wlodawer, A]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:36:24 2008''
+[[Category: Zdanov, A]]
+[[Category: Cytokine]]