4ari: Difference between revisions

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-[[Image:4ari.png|left|200px]]
-{{STRUCTURE_4ari|  PDB=4ari  |  SCENE=  }}
+==Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and the benzoxaborole AN2679 in the editing conformation==
+<StructureSection load='4ari' size='340' side='right'caption='[[4ari]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ari]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARI FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=N79:[(1S,5R,6R,8R)-6-(6-AMINOPURIN-9-YL)SPIRO[2,4,7-TRIOXA-3-BORANUIDABICYCLO[3.3.0]OCTANE-3,9-8-OXA-9-BORANUIDABICYCLO[4.3.0]NONA-1(6),2,4-TRIENE]-8-YL]METHYL+DIHYDROGEN+PHOSPHATE'>N79</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ari FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ari OCA], [https://pdbe.org/4ari PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ari RCSB], [https://www.ebi.ac.uk/pdbsum/4ari PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ari ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SYL_ECOLI SYL_ECOLI]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Leucyl-tRNA synthetase (LeuRS) produces error-free leucyl-tRNA(Leu) by coordinating translocation of the 3' end of (mis-)charged tRNAs from its synthetic site to a separate proofreading site for editing. Here we report cocrystal structures of the Escherichia coli LeuRS-tRNA(Leu) complex in the aminoacylation or editing conformations, showing that translocation involves correlated rotations of four flexibly linked LeuRS domains. This pivots the tRNA to guide its charged 3' end from the closed aminoacylation state to the editing site. The editing domain unexpectedly stabilizes the tRNA during aminoacylation, and a large rotation of the leucine-specific domain positions the conserved KMSKS loop to bind the 3' end of the tRNA, promoting catalysis. Our results give new insight into the structural dynamics of a molecular machine that is essential for accurate protein synthesis.
-===Ternary complex of E. coli leucyl-tRNA synthetase, tRNA(leu) and the benzoxaborole AN2679 in the editing conformation===
+Structural dynamics of the aminoacylation and proofreading functional cycle of bacterial leucyl-tRNA synthetase.,Palencia A, Crepin T, Vu MT, Lincecum TL Jr, Martinis SA, Cusack S Nat Struct Mol Biol. 2012 Jun 10. doi: 10.1038/nsmb.2317. PMID:22683997<ref>PMID:22683997</ref>
-{{ABSTRACT_PUBMED_22683997}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4ari" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[4ari]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARI OCA].
+*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
+*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
-==Reference==
+== References ==
-<ref group="xtra">PMID:022683997</ref><references group="xtra"/>
+<references/>
-[[Category: Escherichia coli]]
+__TOC__
-[[Category: Leucine--tRNA ligase]]
+</StructureSection>
-[[Category: Crepin, T.]]
+[[Category: Escherichia coli K-12]]
-[[Category: Cusack, S.]]
+[[Category: Large Structures]]
-[[Category: Jr, T L.Lincecum.]]
+[[Category: Crepin T]]
-[[Category: Martinis, S A.]]
+[[Category: Cusack S]]
-[[Category: Palencia, A.]]
+[[Category: Lincecum Jr TL]]
-[[Category: Vu, M T.]]
+[[Category: Martinis SA]]
-[[Category: Class i aminoacyl-trna synthetase]]
+[[Category: Palencia A]]
-[[Category: Ligase]]
+[[Category: Vu MT]]
-[[Category: Ligase-rna complex]]
-[[Category: Protein biosynthesis]]