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[[Image:3qt4.png|left|200px]]


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==Structure of digestive procathepsin L 3 of Tenebrio molitor larval midgut==
The line below this paragraph, containing "STRUCTURE_3qt4", creates the "Structure Box" on the page.
<StructureSection load='3qt4' size='340' side='right'caption='[[3qt4]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3qt4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QT4 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PG6:1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE'>PG6</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
{{STRUCTURE_3qt4|  PDB=3qt4  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qt4 OCA], [https://pdbe.org/3qt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qt4 RCSB], [https://www.ebi.ac.uk/pdbsum/3qt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qt4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7YXL2_TENMO Q7YXL2_TENMO]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cathepsin L-like proteinases (CAL) are major digestive proteinases in the beetle Tenebrio molitor. Procathepsin Ls 2 (pCAL2) and 3 (pCAL3) were expressed as recombinant proteins in Escherichia coli, purified and activated under acidic conditions. Immunoblot analyses of different T. molitor larval tissues demonstrated that a polyclonal antibody to pCAL3 recognized pCAL3 and cathepsin L 3 (CAL3) only in the anterior two-thirds of midgut tissue and midgut luminal contents of T. molitor larvae. Furthermore, immunocytolocalization data indicated that pCAL3 occurs in secretory vesicles and microvilli in anterior midgut. Therefore CAL3, like cathepsin L 2 (CAL2), is a digestive enzyme secreted by T. molitor anterior midgut. CAL3 hydrolyses Z-FR-MCA and Z-RR-MCA (typical cathepsin substrates), whereas CAL2 hydrolyses only Z-FR-MCA. Active site mutants (pCAL2C25S and pCAL3C26S) were constructed by replacing the catalytic cysteine with serine to prevent autocatalytic processing. Recombinant pCAL2 and pCAL3 mutants (pCAL2C25S and pCAL3C26S) were prepared, crystallized and their 3D structures determined at 1.85 and 2.1 A, respectively. While the overall structure of these enzymes is similar to other members of the papain superfamily, structural differences in the S2 subsite explain their substrate specificities. The data also supported models for CAL trafficking to lysosomes and to secretory vesicles to be discharged into midgut contents.


===Structure of digestive procathepsin L 3 of Tenebrio molitor larval midgut===
The 3D structure and function of digestive cathepsin L-like proteinases of Tenebrio molitor larval midgut.,Beton D, Guzzo CR, Ribeiro AF, Farah CS, Terra WR Insect Biochem Mol Biol. 2012 May 30. PMID:22659439<ref>PMID:22659439</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3qt4" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 22659439 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_22659439}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
[[3qt4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QT4 OCA].
 
==Reference==
<ref group="xtra">PMID:022659439</ref><references group="xtra"/>
[[Category: Cathepsin L]]
[[Category: Tenebrio molitor]]
[[Category: Tenebrio molitor]]
[[Category: Beton, D.]]
[[Category: Beton D]]
[[Category: Farah, C S.]]
[[Category: Farah CS]]
[[Category: Guzzo, C R.]]
[[Category: Guzzo CR]]
[[Category: Terra, W R.]]
[[Category: Terra WR]]
[[Category: Cysteine proteinase]]
[[Category: Hydrolase]]
[[Category: Insect larval midgut]]
[[Category: Intramolecular dissulphide bond]]
[[Category: Zymogen]]

Latest revision as of 13:22, 6 November 2024

Structure of digestive procathepsin L 3 of Tenebrio molitor larval midgutStructure of digestive procathepsin L 3 of Tenebrio molitor larval midgut

Structural highlights

3qt4 is a 1 chain structure with sequence from Tenebrio molitor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.11Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7YXL2_TENMO

Publication Abstract from PubMed

Cathepsin L-like proteinases (CAL) are major digestive proteinases in the beetle Tenebrio molitor. Procathepsin Ls 2 (pCAL2) and 3 (pCAL3) were expressed as recombinant proteins in Escherichia coli, purified and activated under acidic conditions. Immunoblot analyses of different T. molitor larval tissues demonstrated that a polyclonal antibody to pCAL3 recognized pCAL3 and cathepsin L 3 (CAL3) only in the anterior two-thirds of midgut tissue and midgut luminal contents of T. molitor larvae. Furthermore, immunocytolocalization data indicated that pCAL3 occurs in secretory vesicles and microvilli in anterior midgut. Therefore CAL3, like cathepsin L 2 (CAL2), is a digestive enzyme secreted by T. molitor anterior midgut. CAL3 hydrolyses Z-FR-MCA and Z-RR-MCA (typical cathepsin substrates), whereas CAL2 hydrolyses only Z-FR-MCA. Active site mutants (pCAL2C25S and pCAL3C26S) were constructed by replacing the catalytic cysteine with serine to prevent autocatalytic processing. Recombinant pCAL2 and pCAL3 mutants (pCAL2C25S and pCAL3C26S) were prepared, crystallized and their 3D structures determined at 1.85 and 2.1 A, respectively. While the overall structure of these enzymes is similar to other members of the papain superfamily, structural differences in the S2 subsite explain their substrate specificities. The data also supported models for CAL trafficking to lysosomes and to secretory vesicles to be discharged into midgut contents.

The 3D structure and function of digestive cathepsin L-like proteinases of Tenebrio molitor larval midgut.,Beton D, Guzzo CR, Ribeiro AF, Farah CS, Terra WR Insect Biochem Mol Biol. 2012 May 30. PMID:22659439[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Beton D, Guzzo CR, Ribeiro AF, Farah CS, Terra WR. The 3D structure and function of digestive cathepsin L-like proteinases of Tenebrio molitor larval midgut. Insect Biochem Mol Biol. 2012 May 30. PMID:22659439 doi:10.1016/j.ibmb.2012.04.010

3qt4, resolution 2.11Å

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