3szi: Difference between revisions

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-[[Image:3szi.png|left|200px]]
-<!--
+==Structure of apo shwanavidin (P21 form)==
-The line below this paragraph, containing "STRUCTURE_3szi", creates the "Structure Box" on the page.
+<StructureSection load='3szi' size='340' side='right'caption='[[3szi]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3szi]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_denitrificans_OS217 Shewanella denitrificans OS217]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SZI FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-{{STRUCTURE_3szi|  PDB=3szi  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3szi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3szi OCA], [https://pdbe.org/3szi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3szi RCSB], [https://www.ebi.ac.uk/pdbsum/3szi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3szi ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q12QS6_SHEDO Q12QS6_SHEDO]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Shwanavidin is an avidin-like protein from the marine proteobactrium Shewanella denitrificans, which exhibits an innate dimeric structure while maintaining high affinity towards biotin. A unique residue (Phe-43) from the L3,4 loop and a distinctive disulfide bridge were shown to account for the high affinity towards biotin. Phe-43 emulates the function and position of the critical intermonomeric Trp that characterizes the tetrameric avidins but is lacking in shwanavidin. The 18 copies of the apo monomer revealed distinctive snapshots of L3,4 and Phe-43, providing rare insight into loop flexibility, binding-site accessibility and psychrophilic adaptation. Nevertheless, as in all avidins, shwanavidin also displays high thermostability properties. The unique features of shwanavidin may provide a platform for the design of a long-sought-after monovalent form of avidin, which would be ideal for novel types of biotechnological application.
-===Structure of apo shwanavidin (P21 form)===
+Structural Adaptation of a Thermostable Biotin-Binding Protein in a Psychrophilic Environment.,Meir A, Bayer EA, Livnah O J Biol Chem. 2012 Apr 5. PMID:22493427<ref>PMID:22493427</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_22493427}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3szi" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 22493427 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_22493427}}
-==About this Structure==
-[[3szi]] is a 12 chain structure of [[Avidin]] with sequence from [http://en.wikipedia.org/wiki/Shewanella_denitrificans Shewanella denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZI OCA].
 ==See Also==
-*[[Avidin|Avidin]]
+*[[Avidin 3D structures|Avidin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:022493427</ref><references group="xtra"/>
+__TOC__
-[[Category: Shewanella denitrificans]]
+</StructureSection>
-[[Category: Livnah, O.]]
+[[Category: Large Structures]]
-[[Category: Meir, A.]]
+[[Category: Shewanella denitrificans OS217]]
-[[Category: Avidin]]
+[[Category: Livnah O]]
-[[Category: Biotin]]
+[[Category: Meir A]]
-[[Category: Biotin-binding protein]]
-[[Category: High affinity system]]
-[[Category: Rhizavidin]]
-[[Category: Shwanavidin]]
-[[Category: Streptavidin]]