4epc: Difference between revisions

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-[[Image:4epc.jpg|left|200px]]
-<!--
+==Crystal structure of Autolysin repeat domains from Staphylococcus epidermidis==
-The line below this paragraph, containing "STRUCTURE_4epc", creates the "Structure Box" on the page.
+<StructureSection load='4epc' size='340' side='right'caption='[[4epc]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[4epc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EPC FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4epc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epc OCA], [https://pdbe.org/4epc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4epc RCSB], [https://www.ebi.ac.uk/pdbsum/4epc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4epc ProSAT]</span></td></tr>
-{{STRUCTURE_4epc|  PDB=4epc  |  SCENE=  }}
+</table>
+== Function ==
+[[https://www.uniprot.org/uniprot/ATL_STAEP ATL_STAEP]]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The bifunctional major autolysin Atl plays a key role in staphylococcal cell separation. Processing of Atl yields catalytically active amidase (AM) and glucosaminidase (GL) domains that are each fused to repeating units. The two repeats of AM (R1 and R2) target the enzyme to the septum, where it cleaves murein between dividing cells. We have determined the crystal structure of R2, which reveals that each repeat folds into two half-open beta-barrel subunits. We furthermore demonstrate that lipoteichoic acid serves as a receptor for the repeats, and that this interaction depends on conserved surfaces in each subunit. Small angle X-ray scattering of the mature amidase reveals the presence of flexible linkers separating the AM, R1 and R2 units. Different levels of flexibility for each linker provide mechanistic insights into the conformational dynamics of the full-length protein and the roles of its components in cell wall association and catalysis. Our analysis supports a model in which the repeats direct the catalytic AM domain to the septum, where it can optimally perform the final step of cell division.
-===Crystal structure of Autolysin repeat domains from Staphylococcus epidermidis===
+Ligand-binding properties and conformational dynamics of autolysin repeat domains in staphylococcal cell wall recognition.,Zoll S, Schlag M, Shkumatov AV, Rautenberg M, Svergun DI, Gotz F, Stehle T J Bacteriol. 2012 May 18. PMID:22609916<ref>PMID:22609916</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_22609916}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 4epc" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 22609916 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_22609916}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[4epc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPC OCA].
-==Reference==
-<ref group="xtra">PMID:022609916</ref><references group="xtra"/>
-[[Category: N-acetylmuramoyl-L-alanine amidase]]
 [[Category: Staphylococcus epidermidis]]
-[[Category: Stehle, T.]]
+[[Category: Stehle T]]
-[[Category: Zoll, S.]]
+[[Category: Zoll S]]
-[[Category: Extracellular]]
-[[Category: Hydrolase]]
-[[Category: Sh3b fold]]