4ann: Difference between revisions

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-[[Image:4ann.jpg|left|200px]]
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+==Crystal Structure Staphylococcus aureus ESSB cytoplasmic fragment==
-The line below this paragraph, containing "STRUCTURE_4ann", creates the "Structure Box" on the page.
+<StructureSection load='4ann' size='340' side='right'caption='[[4ann]], [[Resolution|resolution]] 1.05&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[4ann]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ANN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ANN FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.05&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ann FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ann OCA], [https://pdbe.org/4ann PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ann RCSB], [https://www.ebi.ac.uk/pdbsum/4ann PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ann ProSAT]</span></td></tr>
-{{STRUCTURE_4ann|  PDB=4ann  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ESSB_STAA8 ESSB_STAA8] Component of the type VII secretion system (Ess) (Probable). Required for the secretion of EsxA and EsxB (By similarity).[UniProtKB:P0C053]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The type VII protein translocation/secretion system, unique to Gram-positive bacteria is a key virulence determinant in Staphylococcus aureus. We aim to characterise the architecture of this secretion machinery and now describe a study of S. aureus EssB, a 52-kDa bitopic membrane protein essential for secretion of the ESAT-6-family-proteins, the prototypic substrate of Type VII secretion. Full-length EssB was heterologously expressed in Escherichia coli, solubilised from the bacterial membrane, purified to homogeneity and shown to be dimeric. A C-terminal truncation, EssBC, and two soluble fragments termed EssB-N and EssB-C, predicted to occur on either side of the cytoplasmic membrane, have been successfully purified in recombinant form, characterised and together with the full-length protein used in crystallisation trials. EssB-N, the 25 kDa N-terminal cytoplasmic fragment, gave well-ordered crystals and we report the structure, determined by single-wavelength anomalous diffraction (SAD) targeting an SeMet derivative, refined to atomic (1.05 A) resolution. EssB-N is dimeric in solution but crystallises as a monomer and displays a fold composed of two globular domains separated by a cleft. The structure is related to that of Ser/Thr protein kinases and our study identifies that the type VII secretion system exploits and re-uses a stable modular entity and fold that has evolved to participate in protein-protein interactions in a similar fashion to the catalytically inert pseudokinases.
-===Crystal Structure Staphylococcus aureus ESSB cytoplasmic fragment===
+Characterisation of Staphylococcus aureus EssB, an integral membrane component of the Type VII secretion system; atomic resolution crystal structure of the cytoplasmic segment.,Zoltner M, Fyfe PK, Palmer T, Hunter WN Biochem J. 2012 Oct 25. PMID:23098276<ref>PMID:23098276</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-[[4ann]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_nctc_8325 Staphylococcus aureus subsp. aureus nctc 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ANN OCA].
+<div class="pdbe-citations 4ann" style="background-color:#fffaf0;"></div>
-[[Category: Staphylococcus aureus subsp. aureus nctc 8325]]
+== References ==
-[[Category: Fyfe, P.]]
+<references/>
-[[Category: Hunter, W N.]]
+__TOC__
-[[Category: Palmer, T.]]
+</StructureSection>
-[[Category: Zoltner, M.]]
+[[Category: Large Structures]]
-[[Category: Ess type vii secretion system]]
+[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-[[Category: Membrane protein]]
+[[Category: Fyfe PK]]
-[[Category: Membrane secretion]]
+[[Category: Hunter WN]]
-[[Category: Virulence]]
+[[Category: Palmer T]]
+[[Category: Zoltner M]]